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Journal of Toxicology
Volume 2015, Article ID 535490, 6 pages
http://dx.doi.org/10.1155/2015/535490
Research Article

Evaluation of Possible Genotoxic Activity of Dirithromycin in Cultured Human Lymphocytes

1Department of Biology, Science and Arts Faculty, Kahramanmaraş Sütçü İmam University, Kahramanmaraş, Turkey
2Department of Biology, Science and Arts Faculty, Mustafa Kemal University, Antakya, Turkey

Received 13 July 2015; Accepted 7 October 2015

Academic Editor: Orish Ebere Orisakwe

Copyright © 2015 Ahmet Kayraldız et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Dirithromycin antibiotic is a 14-membered lactone ring macrolide and is widely used in medicine to treat many different types of bacterial infections. In the present study, the possible genotoxicity of dirithromycin was evaluated in cultured human lymphocytes by using sister chromatid exchanges (SCEs), chromosome aberration (CA), and micronucleus (MN) tests and also cell proliferation kinetics such as mitotic index (MI), replication index (RI), and nuclear division index (NDI) were analyzed for cytotoxicity. Cell cultures were treated with four different concentrations of dirithromycin (37.75, 67.50, 125, and 250 µg/mL) for 24 and 48 h periods. Dirithromycin significantly induced SCE and MN frequency at all concentrations in both 24 and 48 h treated cells. In addition, CA level has been markedly increased in the cells treated with almost all concentrations of dirithromycin for 24 (except 37.75 µg/mL) and 48 h treatment periods as compared to control. However, MI, RI, and NDI values were not affected by the dirithromycin treatment (p > 0.05). The results of this study indicated that dirithromycin treatment caused genetic damage by increasing the level of cytogenetic endpoints, suggesting its genotoxic and mutagenic action on human lymphocytes in vitro.