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Journal of Toxicology
Volume 2016, Article ID 8515089, 7 pages
Research Article

Comparisons of Curative Effects of Chlorophyll from Sauropus androgynus (L) Merr Leaf Extract and Cu-Chlorophyllin on Sodium Nitrate-Induced Oxidative Stress in Rats

1Department of Biology, Faculty of Medicine, Universitas Islam Sultan Agung, Semarang 50112, Indonesia
2Department of Clinical Nutrition, Faculty of Medicine, Universitas Islam Sultan Agung, Semarang 50112, Indonesia
3Sultan Agung Islamic Hospital, Semarang 50112, Indonesia
4Master Program of Biology, Satya Wacana Christian University, Jl. Diponegoro 52-60, Salatiga 50711, Indonesia

Received 3 August 2016; Accepted 24 October 2016

Academic Editor: Orish Ebere Orisakwe

Copyright © 2016 Suparmi Suparmi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Sodium nitrate (NaNO2) widely used as food additive for coloring and preserving meat has been reported to induce oxidative stress and cause histopathologic changes, nitrosative tissue damage, and lipid peroxidation in liver and kidney. Therefore, the present study compared the curative effect of chlorophyll from Sauropus androgynus (L) Merr and Cu-chlorophyllin as antioxidant in NaNO2-induced female Wistar rats based on haematological, serum biochemical, and histological evaluation. Thirty male Wistar rats were randomly assigned into six groups of five rats each. NaNO2 were given at a subacute dose of 50 mg/kg bw intraperitoneally for 10 days. Chlorophyll from S. androgynus and Cu-chlorophyllin from K-Liquid™ were given in the following 14 days at the two doses: 0,016 mg/mL and 0.008 mg/mL. NaNO2 exposure resulted in significant reductions () in values of packed cell volume (PCV), haemoglobin (Hb) concentration and red blood cell (RBC) count, transferrin, and ferritin and elevation in malondialdehyde (MDA) level and schistocytes percentage with insignificant reductions in serum albumin and transferrin levels. Histology of kidney and liver were changed insignificantly () to normal values. Chlorophyll from S. androgynus and Cu-chlorophyllin possess antioxidant potentials to protect against toxicities induced by sodium nitrate.