Screening of Pesticides with the Potential of Inducing DSB and Successive Recombinational Repair
Table 2
Percentage of cells with -H2AX foci due to treatments.
Compound
Doses (microM)
Mean of % cells (S.D)
Median of % cells with total damage
(chi-squared value)
Without foci
With 1 to 10 foci
With more than 10 foci
With total damaged
Glyphosate
0
48.33 (2.94)
51.33 (3.33)
0.33 (0.52)
51.67 (2.94)
51.50
0.0107 (13.13)
0.4
31.17 (3.76)
67.17 (3.66)
1.67 (1.37)
68.83 (3.76)
69.00a
2
33.17 (4.07)
57.50 (10.62)
9.33 (7.94)
66.83 (4.07)
65.50
10
30.17 (13.21)
61.00 (10.18)
8.83 (4.36)
69.83 (13.21)
75.00a
50
33.67 (4.50)
63.17 (4.71)
3.17 (3.71)
66.33 (4.50)
67.50
Etoposide
50
3.50 (1.42)
6.83 (4.71)
89.67 (5.20)
96.50 (1.52)
96.50a
Paraoxon
0
78.00 (10.12)
21.00 (8.92)
1.00 (1.67)
22.00 (10.12)
21.00
0.0054 (14.68)
0.2
57.33 (17.28)
40.67 (15.93)
2.00 (1.79)
42.67 (17.28)
41.00
1
43.33 (13.31)
52.33 (12.23)
4.33 (3.67)
56.67 (13.31)
56.00a
5
52.00 (5.22)
43.33 (5.47)
4.67 (3.72)
48.00 (5.22)
48.00
25
42.33 (15.92)
43.67 (24.57)
14.00 (12.71)
57.67 (15.92)
60.00a
Etoposide
50
7.00 (4.86)
4.33 (2.34)
88.67 (5.32)
93.00 (4.86)
91.00b
Permethrin
0
55.33 (6.15)
42.67 (6.15)
2.00 (0.00)
44.67 (6.15)
47.00
0.0092 (13.46)
8
29.00 (16.19)
65.67 (13.65)
5.33 (5.75)
71.00 (16.19)
73.00a
40
36.00 (6.33)
61.00 (5.90)
3.00 (2.10)
64.00 (6.33)
64.00
200
24.33 (11.13)
70.33 (7.31)
5.33 (6.02)
75.67 (11.13)
76.00a
1000
36.33 (18.82)
60.00 (16.44)
3.67 (2.94)
63.67 (18.82)
64.00
Etoposide
50
0.33 (0.82)
7.00 (3.29)
92.67 (3.01)
99.67 (0.82)
100.00b
Endosulfan lactone
0
55.33 (6.15)
42.67 (6.15)
2.00 (0.00)
44.67 (6.15)
47.00
0.0001 (23.30)
0.08
32.67 (5.61)
62.33 (7.31)
5.00 (4.52)
67.33 (5.61)
66.00
0.4
27.00 (5.33)
70.33 (4.46)
2.67 (2.42)
73.00 (5.33)
74.00a
2
27.00 (5.33)
73.67 (3.88)
3.33 (2.73)
77.00 (3.03)
77.00a
10
47.00 (8.08)
51.67 (4.53)
1.33 (1.63)
53.00 (8.07)
53.00
Etoposide
50
0.33 (0.82)
7.00 (3.27)
92.67 (3.01)
99.67 (0.82)
100.00b
PCP
0
78.00 (10.12)
21.00 (8.92)
1.00 (1.67)
22.00 (10.12)
21.00
0.0259 (11.06)
0.03
48.33 (14.11)
49.00 (13.19)
2.67 (1.63)
51.67 (14.11)
52.00
0.15
39.67 (16.71)
56.33 (17.45)
4.00 (2.83)
60.33 (16.71)
60.00a
0.75
62.67 (23.31)
34.00 (22.52)
3.33 (2.73)
37.33 (23.31)
30.00
3.75
68.67 (23.45)
29.00 (23.07)
2.33 (2.34)
31.33 (23.45)
31.00
Etoposide
50
7.00 (4.86)
4.33 (2.34)
88.67 (5.32)
93.00 (4.86)
91.00b
Kruskal-Wallis test considering all damaged cells; d.f. 4 in all tests; significant dose compared with the negative control; post hoc Dunn’s test, . Etoposide results were always significant: Mann–Whitney test, . PCP: pentachlorophenol; only compounds with a positive result are presented here considering a value of <0.05 for significance. See text.
