Screening of Pesticides with the Potential of Inducing DSB and Successive Recombinational Repair
Table 4
Detection of Ku80 phosphorylated and Rad51 proteins in mononuclear cells treated with pesticides and metabolites that induced -H2AX foci.
Compound
Dose (µM)
P-Ku80
Rad51
Mean OD (SD)
Median OD
(chi-squared value)
Mean OD (SD)
Median OD
(chi-squared value)
Glyphosate
0
100.00 (34.48)
104.05
0.0297 (7.985)
100.00 (47.93)
104.17
0.4955 (2.559)
1.25
200.0 (63.99)
206.20
134.4 (34.57)
122.89
2.5
227.3 (98.69)
190.91
140.8 (41.92)
158.29
5
253.9 (130.40)
205.2
107.5 (49.28)
113.41
Etoposide
214.2 (48.55)
198.21
0.028
119.3 (11.74)
84.26
0.6571
Paraoxon
0
100.0 (22.11)
102.55
0.0077 (9.551)
100.0 (50.54)
96.74
0.3812 (3.243)
1
186.64 (39.72)
196.4
178.8 (73.36)
171.4
5
155.32 (17.06)
159.1
115.9 (6.84)
113.0
25
154.58 (8.76)
157.7
180.6 (95.02)
147.7
Etoposide
155.4 (63.53)
167.30
0.1044
109.5 (25.86)
98.03
0.6571
Kruskal-Wallis test: P-Ku80 was significant in glyphosate and paraoxon; ; d.f. 3 in all tests; significant dose compared with the negative control; post hoc Dunn’s test: ; Mann–Whitney test: ; OD: optical density. The results presented correspond to two separate experiments; only compounds who were positive are presented. See text.
