Long-Term Safety of PEGylated Coagulation Factor VIII in the Immune-Deficient Rowett Nude Rat

Figure 2

Immunohistochemical detection of polyethylene glycol (PEG) in (a) macrophages in subcutaneous tissue from a rat injected subcutaneously with 40 kDa PEG (positive control), (b) choroid plexus from rat dosed with 0 IU N8-GP/kg every fourth day for 52 weeks (negative control), and (c) choroid plexus from a rat dosed with 1200 IU N8-GP/kg every fourth day for 52 weeks. In (a), fluorescent green staining represents PEG content in macrophages. In (b) and (c), green staining represents autofluorescence staining of red blood cells in the core of the choroid plexus. Red staining in (a), (b), and (c) represents nuclei.

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