Research Article
Tramadol Induced Adrenal Insufficiency: Histological, Immunohistochemical, Ultrastructural, and Biochemical Genetic Experimental Study
Table 1
Details giving primer sequences, expected product sizes, and annealing temperature for the genes amplified.
| Gene | Primer sequence (5-3) | Product size | Annealing temperature |
| GPX | Forward GGAGAATGGCAAGAATGAAGA | 139 pb | 55°C | Reverse CCGCAGGAAGGTAAAGAG | B-actin | Forward GAGAGGGAAATCGTGCGTGAC | 452 pb | 55°C | Reverse CATCTGCTGGAAGGTGGACA | Thioredoxin reductase | Forward CCGCAACAGCCAAAATGGTGA | 339 pb | 60°C | Reverse AGCATGATTAGGCAAACTCCGTAA | 18s | Forward TTGACGGAAGGGCACCACCA | 13 pb | 55°C | Reverse GCACCACCACCCACGGAATCG |
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