Research Article

Reactive Oxygen Species Generation in Human Cells by a Novel Magnetic Resonance Imaging Contrast Agent

Figure 2

Cell morphology after various treatment conditions. Human prostate cancer cells (PC3), human normal tongue cells (Hs-680Tg), and human head and neck cancer cells (HN5) were treated with the greatest clinical exposure concentration of the novel MRI Co : NAC positive-contrast agent for C4 marker or its components (1% [w/v] CoCl2⋅6H2O, 2% [w/v] N-acetylcysteine (NAC), or the combined Co : NAC solutions (1% : 2% [w/v])). At 90 minutes after treatment, cell morphology pictures were taken with a bright field Evos XL core microscope (AMEX 1000, Life Technologies, Carlsbad, CA, USA) at ×10 magnification. Controls (Con) were untreated cells.