An Assessment of the Genotoxicity and Subchronic Toxicity of a Supercritical Fluid Extract of the Aerial Parts of Hemp
Table 11
Summary of selected† clinical chemistry findings in the 90-day repeated oral toxicity study.
Group
ALT
AST
GGT
ALP
TBIL
CREA
CHOL
Pi
ALB
TPROT
(mg/kg bw/day)
U/L
U/L
U/L
U/L
μmol/L
μmol/L
mmol/L
mmol/L
g/L
g/L
Male (main groups, = 10 each)
Control
65.11 ± 15.73
98.99 ± 17.04
1.21 ± 0.23
129.10 ± 35.04
1.93 ± 0.43
28.84 ± 2.54
1.90 ± 0.22
1.93 ± 0.24
34.81 ± 0.60
61.99 ± 2.53
100
52.96 ± 9.36
95.25 ± 9.39
1.27 ± 0.36
124.80 ± 40.28
1.52 ± 0.25
27.83 ± 2.98
1.86 ± 0.38
2.26 ± 0.53
35.38 ± 1.15
64.65 ± 3.49
360
62.35 ± 16.31
83.03 ± 6.33
2.11 ± 0.67∗∗
129.90 ± 43.44
1.70 ± 0.24
29.01 ± 2.29
1.84 ± 0.26
2.38 ± 0.34
36.11 ± 1.41
66.90 ± 5.38
720
51.50 ± 11.56
88.31 ± 11.96
3.44 ± 1.06∗∗
161.60 ± 38.08
1.87 ± 0.33
29.27 ± 3.56
1.68 ± 0.42
2.10 ± 0.37
36.47 ± 1.78∗∗
67.29 ± 4.40
Historical range♮
28.0–86.2
67.9–135.7
0.0–2.2
56–184
0.71–2.79
18.5 ± 37.2
1.27–2.44
1.40–2.41
33.0–37.4
56.8–68.0
Male (recovery groups, = 5 each)
Control
57.56 ± 15.48
89.22 ± 11.97
0.98 ± 0.18
104.60 ± 27.83
1.61 ± 0.30
30.42 ± 2.46
2.22 ± 0.29
1.95 ± 0.37
35.38 ± 0.88
66.48 ± 3.33
720
48.70 ± 17.40
76.78 ± 11.47
1.48 ± 0.16∗∗
89.00 ± 16.00
1.78 ± 0.41
30.56 ± 4.48
1.82 ± 0.42
2.08 ± 0.28
36.00 ± 0.99
65.58 ± 3.27
Female (main groups, = 10 each)
Control
52.32 ± 7.80
85.88 ± 10.45
1.54 ± 0.29
55.80 ± 16.72
1.78 ± 0.22
31.81 ± 2.69
1.94 ± 0.38
1.59 ± 0.23
35.35 ± 1.23
62.16 ± 2.61
100
51.04 ± 10.64
91.17 ± 22.32
1.35 ± 0.47
64.70 ± 26.19
1.91 ± 0.39
30.36 ± 2.30
1.80 ± 0.27
1.96 ± 0.36
36.18 ± 1.50
64.78 ± 2.63
360
51.40 ± 8.64
84.86 ± 12.67
3.11 ± 0.74∗∗
72.70 ± 22.96
1.60 ± 0.25
30.85 ± 3.33
2.19 ± 0.32
1.99 ± 0.35∗∗
36.33 ± 1.21
64.62 ± 2.83
720
58.11 ± 11.93
85.12 ± 15.40
7.41 ± 1.72∗∗
91.10 ± 23.86∗∗
2.13 ± 0.69
30.37 ± 2.35
2.71 ± 0.41∗∗
1.84 ± 0.15∗∗
35.90 ± 1.84
65.15 ± 4.63
Historical range♮
23.4–87.7
71.0–141.8
0.0–2.9
25.0–126
1.23–3.30
25.6–40.8
1.35–3.39
0.93–2.01
33.2–41.0
57.1–74.5
Female (recovery groups, = 5 each)
Control
48.12 ± 13.64
73.58 ± 11.26
1.06 ± 0.15
53.60 ± 13.94
2.03 ± 0.28
34.44 ± 2.50
2.37 ± 0.50
1.39 ± 0.32
37.28 ± 2.70
68.94 ± 5.95
720
38.40 ± 4.83
70.44 ± 10.04
1.96 ± 0.46∗∗
49.60 ± 10.36
1.78 ± 0.24
30.72 ± 2.12
2.22 ± 0.31
1.22 ± 0.28
36.76 ± 1.09
66.44 ± 1.28
Data represent the mean values and the standard deviation. †: only parameters with statistically significant findings are shown in table. and ∗∗P < 0.01, statistical significances were determined with Duncan's multiple range test or with Mann–Whitney test versus control. ♮: minimum and maximum levels reported as the range of historical control values.