An Assessment of the Genotoxicity and Subchronic Toxicity of a Supercritical Fluid Extract of the Aerial Parts of Hemp
Table 13
Summary of relative organ weights (organ weight relative to body weight) (%) in the 90-day repeated oral toxicity study.
Group (mg/kg bw/day)
Brain
Liver
Kidneys
Heart
Thymus
Spleen
Testes/uterus
Epididymides/ovaries
Adrenals
Thyroid
Male (main groups, = 10 each)
Control
0.539 ± 0.028
2.515 ± 0.098
0.554 ± 0.036
0.284 ± 0.018
0.103 ± 0.021
0.165 ± 0.026
0.902 ± 0.051
0.441 ± 0.052
0.018 ± 0.002
0.006 ± 0.002
100
0.545 ± 0.054
2.595 ± 0.081
0.570 ± 0.040
0.281 ± 0.034
0.094 ± 0.012
0.157 ± 0.011
0.933 ± 0.054
0.426 ± 0.038
0.019 ± 0.003
0.005 ± 0.002
360
0.581 ± 0.043
3.196 ± 0.147∗∗
0.591 ± 0.044
0.272 ± 0.019
0.090 ± 0.024
0.158 ± 0.025
0.938 ± 0.086
0.430 ± 0.049
0.023 ± 0.004∗∗
0.006 ± 0.001
720
0.617 ± 0.055∗∗
4.093 ± 0.228∗∗
0.631 ± 0.027∗∗
0.290 ± 0.028
0.088 ± 0.020
0.168 ± 0.021
1.002 ± 0.080∗∗
0.414 ± 0.026
0.024 ± 0.004∗∗
0.007 ± 0.002
Historical range♮
0.441–0.599
1.916–3.108
0.466–0.650
0.216–0.311
0.057–0.191
0.058–0.219
0.642–1.011
0.320–0.512
0.012–0.023
0.0037–0.0089
Male (recovery group, = 5 each)
Control
0.478 ± 0.015
2.454 ± 0.089
0.522 ± 0.019
0.236 ± 0.015
0.087 ± 0.015
0.158 ± 0.019
0.806 ± 0.014
0.418 ± 0.027
0.017 ± 0.002
0.006 ± 0.000
720
0.550 ± 0.032∗∗
2.392 ± 0.158
0.587 ± 0.037∗∗
0.265 ± 0.020
0.098 ± 0.021
0.168 ± 0.023
0.941 ± 0.082∗∗
0.460 ± 0.032
0.017 ± 0.001
0.007 ± 0.000∗∗
Female (main groups, = 10 each)
Control
0.825 ± 0.028
2.693 ± 0.174
0.625 ± 0.040
0.322 ± 0.022
0.128 ± 0.018
0.230 ± 0.034
0.327 ± 0.088
0.0669 ± 0.0110
0.0348 ± 0.0043
0.0096 ± 0.0022
100
0.805 ± 0.072
2.854 ± 0.133
0.609 ± 0.024
0.324 ± 0.027
0.159 ± 0.024∗∗
0.196 ± 0.023∗∗
0.251 ± 0.051
0.0616 ± 0.0162
0.0357 ± 0.0051
0.0083 ± 0.0018
360
0.853 ± 0.094
3.298 ± 0.144∗∗
0.598 ± 0.034
0.317 ± 0.033
0.145 ± 0.026
0.191 ± 0.023∗∗
0.224 ± 0.094∗∗
0.0627 ± 0.0108
0.0413 ± 0.0054
0.0091 ± 0.0027
720
0.882 ± 0.055
4.322 ± 0.281∗∗
0.637 ± 0.038
0.323 ± 0.025
0.141 ± 0.017
0.169 ± 0.018∗∗
0.215 ± 0.055∗∗
0.0653 ± 0.0163
0.0469 ± 0.0068∗∗
0.0109 ± 0.0037
Historical range♮
0.681–0.943
2.172–3.214
0.530–0.752
0.273–0.396
0.093–0.217
0.140–0.298
0.167–0.852
0.043–0.086
0.026–0.044
0.0052–0.0128
Female (recovery group, = 5 each)
Control
0.820 ± 0.023
2.671 ± 0.167
0.633 ± 0.032
0.299 ± 0.012
0.151 ± 0.020
0.196 ± 0.020
0.290 ± 0.045
0.0562 ± 0.0137
0.0285 ± 0.0031
0.0090 ± 0.0004
720
0.885 ± 0.043
2.700 ± 0.064
0.629 ± 0.014
0.309 ± 0.016
0.152 ± 0.008
0.197 ± 0.023
0.221 ± 0.047
0.0600 ± 0.0136
0.0345 ± 0.0027
0.0109 ± 0.0010∗∗
Data represent the mean values and the standard deviation. and ∗∗P < 0.01, statistical significances were determined with Duncan's multiple range test or with Mann–Whitney test versus control. ♮: minimum and maximum levels reported as the range of historical control values.