An Assessment of the Genotoxicity and Subchronic Toxicity of a Supercritical Fluid Extract of the Aerial Parts of Hemp
Table 14
Summary of relative organ weights (organ and body weight relative to brain weight) (%) in the 90-day repeated oral toxicity study.
Group (mg/kg bw/day)
Body weight
Liver
Kidneys
Heart
Thymus
Spleen
Testes/uterus
Epididymides/ovaries
Adrenals
Thyroid
Male (main groups, = 10 each)
Control
18591.6 ± 968.39
468.24 ± 40.18
102.89 ± 6.65
52.69 ± 3.69
19.15 ± 3.63
30.70 ± 4.40
167.49 ± 8.20
81.70 ± 8.27
3.31 ± 0.39
1.09 ± 0.28
100
18490.1 ± 1785.06
479.69 ± 46.21
105.21 ± 11.56
51.68 ± 5.97
17.48 ± 3.71
28.94 ± 3.10
172.03 ± 13.70
78.46 ± 6.78
3.57 ± 0.45
1.01 ± 0.34
360
17293.5 ± 1365.76
552.65 ± 49.26∗∗
102.35 ± 11.99
46.98 ± 5.17
15.64 ± 4.23
27.28 ± 4.46
161.87 ± 16.34
74.05 ± 7.67
4.02 ± 0.61∗∗
1.11 ± 0.22
720
16340.7 ± 1594.47∗∗
670.06 ± 90.43∗∗
103.09 ± 10.65
47.16 ± 3.98
14.29 ± 3.43
27.46 ± 4.11
163.26 ± 16.05
67.57 ± 6.46∗∗
3.96 ± 0.50∗∗
1.14 ± 0.25
Historical range♮
16681–22689
371.50–660.20
91.59–131.25
43.53–65.22
10.45–38.18
11.82–41.71
129.65–186.05
63.76–98.40
2.19–4.32
0.78–1.80
Male (recovery group, = 5 each)
Control
20923.4 ± 682.77
513.36 ± 19.85
109.06 ± 3.33
49.28 ± 2.09
18.12 ± 3.19
32.97 ± 3.96
168.56 ± 3.71
87.37 ± 4.30
3.44 ± 0.34
1.19 ± 0.07
720
18234.7 ± 1067.39∗∗
435.79 ± 31.87∗∗
106.81 ± 1.71
48.18 ± 2.34
17.79 ± 3.54
30.55 ± 3.78
171.55 ± 16.96
83.77 ± 5.21
3.07 ± 0.23
1.25 ± 0.14
Female (main groups, = 10 each)
Control
12135.2 ± 419.40
326.63 ± 20.94
75.83 ± 6.20
39.04 ± 3.11
15.47 ± 2.16
27.91 ± 3.98
39.68 ± 10.63
8.11 ± 1.30
4.22 ± 0.51
1.16 ± 0.27
100
12513.6 ± 1162.30
357.88 ± 44.84
76.19 ± 6.93
40.48 ± 4.55
19.89 ± 3.29∗∗
24.50 ± 3.34
31.50 ± 6.98
7.74 ± 2.23
4.50 ± 0.95
1.04 ± 0.25
360
11848.4 ± 1337.88
391.14 ± 50.90∗∗
70.90 ± 9.89
37.63 ± 6.71
17.29 ± 4.20
22.74 ± 4.47∗∗
26.75 ± 12.08∗∗
7.46 ± 1.70
4.89 ± 0.79
1.06 ± 0.23
720
11377.7 ± 735.76
490.87 ± 33.83∗∗
72.47 ± 6.17
36.65 ± 3.17
15.97 ± 2.06
19.19 ± 2.33∗∗
24.65 ± 6.98∗∗
7.41 ± 1.83
5.32 ± 0.70∗∗
1.24 ± 0.44
Historical range♮
10600.0–14685.7
276.04–412.95
65.08–104.71
33.65–49.74
11.82–27.55
17.84–38.34
18.96–99.52
5.19–10.79
3.12–5.49
0.65–1.53
Female (recovery group, = 5 each)
Control
12200.5 ± 339.93
325.87 ± 21.65
77.26 ± 4.22
36.44 ± 2.25
18.49 ± 2.75
23.86 ± 1.79
35.34 ± 4.81
6.84 ± 1.58
3.47 ± 0.38
1.10 ± 0.06
720
11316.1 ± 560.61
305.45 ± 14.34
71.15 ± 3.85
35.03 ± 2.81
17.19 ± 1.42
22.18 ± 1.86
24.95 ± 5.06
6.78 ± 1.55
3.90 ± 0.25
1.23 ± 0.11
Data represent the mean values and the standard deviation. and ∗∗P < 0.01, statistical significances were determined with Duncan's multiple range test or with Mann–Whitney test versus control. ♮: minimum and maximum levels reported as the range of historical control values.
