Table 2: Summary table of the results of the confirmatory mutation test.

Confirmatory mutation test (preincubation test)

Concentrations (g/plate)Salmonella typhimurium tester strainsEscherichia coli
TA 98TA 100TA 1535TA 1537WP2uvrA
-S9+S9-S9+S9-S9+S9-S9+S9-S9+S9

Mean values of revertants per plate and mutation rate (MR)MeanMRMeanMRMeanMRMeanMRMeanMRMeanMRMeanMRMeanMRMeanMRMeanMR

Untreated control14.70.9622.71.33102.01.02126.31.108.70.938.00.839.31.279.00.8413.30.7719.31.16
DMSO control15.31.0017.01.00100.01.00114.31.009.31.009.71.007.31.0010.71.0017.31.0016.71.00
Ultrapure water control86.71.0013.01.0017.71.00

50005.00.3316.70.9844.30.4461.30.540.30.049.00.930.70.094.70.4412.70.7316.30.98
16009.00.5923.31.3746.70.4771.30.622.70.298.30.862.00.278.70.8116.30.9417.71.06
5008.30.5418.01.0645.70.4669.70.616.00.649.30.970.70.098.30.7814.30.8315.30.92
16012.30.8026.71.5780.30.8091.00.808.30.8910.01.034.30.599.30.8810.70.6220.31.22
5012.30.8026.31.5594.70.95100.70.8810.01.0710.01.037.71.0510.30.9716.00.9218.01.08
1614.00.9129.01.7194.70.95101.00.889.31.008.70.907.71.0511.71.0912.30.7119.31.16
514.30.9323.01.3594.70.95115.71.0110.31.1113.01.347.31.0011.31.0616.00.9218.31.10

NPD (4)310.020.22
SAZ (2)1389.316.033104.0238.77
9AA (50)586.079.91
MMS (2 μL)898.750.87
2AA (2)989.358.201752.015.32113.311.72133.712.53
2AA (50)181.310.88

DMSO was applied as vehicle of the test article and positive control substances: NPD, 9AA, and 2AA; and the ultrapure water was applied as vehicle for the SAZ and MMS. The mutation rate of the test item and the untreated control is given referring to the DMSO.