An Assessment of the Genotoxicity and Subchronic Toxicity of a Supercritical Fluid Extract of the Aerial Parts of Hemp
Table 5
Summary of selected† hematological findings in the 14-day repeated oral toxicity study.
Group
WBC
NEU
LYM
MONO
EOS
RBC
HCT
MCV
MCH
MCHC
PLT
RET
(mg/kg bw/day)
×109/L
%
%
%
%
×1012/L
L/L
fL
pg
g/L
×109/L
%
Male (main groups, = 5 each unless otherwise noted)
Control
7.56 ± 1.03
14.90 ± 1.96
82.56 ± 2.13
1.78 ± 0.78
0.74 ± 0.34
8.17 ± 0.71
0.44 ± 0.04
53.70 ± 2.47
19.06 ± 0.78
355.20 ± 5.93
773.40 ± 66.89
4.74 ± 0.58
1000
8.20 ± 1.54
15.60 ± 3.16
82.20 ± 3.09
1.66 ± 0.51
0.46 ± 0.15
8.59 ± 0.54
0.45 ± 0.02
52.98 ± 2.12
19.02 ± 0.74
360.20 ± 2.59
868.00 ± 47.65
2.96 ± 0.39∗∗
2000
7.03 ± 1.40
9.92 ± 4.69
88.90 ± 5.21
1.06 ± 0.39
0.12 ± 0.16∗∗
8.92 ± 0.72
0.45 ± 0.01
50.62 ± 3.30
18.28 ± 0.87
361.80 ± 7.60
901.80 ± 93.29
2.51 ± 1.30∗∗
3000††
14.44 ± 2.59∗∗
13.35 ± 1.91
82.90 ± 2.97
3.35 ± 1.20
0.30 ± 0.14
9.17 ± 0.06
0.44 ± 0.02
48.30 ± 1.84
18.40 ± 0.28
380.50 ± 9.19∗∗
1009.50 ± 44.55∗∗
1.49 ± 1.22∗∗
Historical range♮
6.23–13.73
6.8–28.2
68.5–91.6
0.8–3.7
0.1–2.2
6.6–9.5
0.37–0.50
48.7–60.8
17.9–21.1
348–377
631–1185
3.35–6.73
Female (main groups, = 5 each unless otherwise noted)
Control
6.21 ± 1.16
10.46 ± 2.87
88.02 ± 3.06
1.02 ± 0.33
0.50 ± 0.22
8.10 ± 0.22
0.44 ± 0.01
54.14 ± 0.90
19.40 ± 0.33
358.60 ± 3.21
913.40 ± 37.32
4.62 ± 0.82
1000
7.24 ± 3.22
10.36 ± 3.69
88.06 ± 4.01
0.94 ± 0.30
0.62 ± 0.22
8.61 ± 0.34
0.46 ± 0.01
52.98 ± 2.57
18.90 ± 0.75
356.80 ± 5.22
887.00 ± 109.73
3.59 ± 0.53
2000
7.31 ± 2.77
13.26 ± 8.36
85.28 ± 8.22
1.12 ± 0.41
0.30 ± 0.10
8.76 ± 0.28
0.45 ± 0.01
51.10 ± 1.08
18.38 ± 0.36∗∗
359.60 ± 7.16
914.60 ± 87.37
3.44 ± 0.92
3000††
4.13 ± 1.49
26.35 ± 20.86
72.20 ± 21.78
1.45 ± 0.92
0.00 ± 0.00∗∗
8.26 ± 0.63
0.42 ± 0.02
50.35 ± 1.20
17.95 ± 0.07∗∗
357.00 ± 9.90
1551.00 ± 599.63
5.58 ± 5.82
Historical range♮
4.07–10.67
5.5–24.2
71.7–92.5
0.7–3.5
0.2–2.7
7.8–9.1
0.41–0.47
48.1–57.8
17.7–20.5
353–374
635–1118
2.56–6.97
Data represents the mean values and the standard deviation. Dose was reduced from 4000 to 3000 mg/kg bw/d on day 2. : only parameters with statistically significant findings are shown in table. : dose was reduced from 4000 to 3000 mg/kg bw/d on day 2. and ∗∗P < 0.01, significance determined with Duncan's multiple range test or with Mann–Whitney test versus control. ♮: minimum and maximum levels reported as the range of historical control values.