An Assessment of the Genotoxicity and Subchronic Toxicity of a Supercritical Fluid Extract of the Aerial Parts of Hemp
Table 8
(a) Summary of mean body weight gain in the 90-day repeated oral toxicity study
Group (mg/kg bw/day)
Body weight gain (g) between days
0-3
3-7
7-10
10-14
14-17
17-21
21-24
24-28
28-35
35-42
42-49
49-56
56-63
63-70
70-77
77-84
84-89
0–89
Males
Control
19.5 ± 2.3
24.3 ± 3.0
18.3 ± 1.4
19.2 ± 3.7
14.9 ± 2.7
14.4 ± 3.0
11.3 ± 2.8
12.3 ± 2.8
20.7 ± 2.7
12.9 ± 2.9
13.3 ± 3.8
8.7 ± 3.5
8.8 ± 2.8
10.2 ± 2.9
6.1 ± 3.5
11.3 ± 3.6
0.2 ± 2.3
226.5 ± 24.5
100
19.3 ± 3.2
23.6 ± 4.3
16.9 ± 4.4
19.5 ± 6.1
15.2 ± 3.9
14.2 ± 4.6
12.3 ± 3.2
11.6 ± 3.3
20.5 ± 5.3
12.4 ± 2.5
11.5 ± 4.9
5.8 ± 2.9
7.0 ± 5.6
8.4 ± 2.6
1.6 ± 3.7∗∗
10.7 ± 2.8
3.6 ± 3.4
214.1 ± 40.6
360
9.6 ± 4.8∗∗
18.7 ± 2.9∗∗
15.7 ± 3.3
15.8 ± 2.7
13.9 ± 3.0
12.8 ± 2.2
10.7 ± 2.3
10.5 ± 1.9
17.5 ± 3.2
12.4 ± 4.0
9.6 ± 5.1
4.9 ± 4.9
8.8 ± 2.6
9.5 ± 3.7
3.0 ± 2.7
12.2 ± 4.5
1.7 ± 5.0
187.3 ± 20.2∗∗
720
3.9 ± 3.5∗∗
17.9 ± 4.5∗∗
12.3 ± 5.9∗∗
16.9 ± 3.1
10.9 ± 3.7∗∗
13.7 ± 3.6
9.4 ± 2.7
10.5 ± 2.1
14.9 ± 4.3∗∗
12.9 ± 4.0
8.7 ± 2.8∗∗
7.9 ± 3.2
6.3 ± 3.0
8.4 ± 2.7
1.1 ± 2.5∗∗
12.5 ± 3.6
1.3 ± 2.8
169.7 ± 15.0∗∗
Females
Control
10.8 ± 2.4
12.1 ± 1.5
8.5 ± 3.2
8.6 ± 3.7
8.9 ± 2.9
6.7 ± 2.0
7.1 ± 3.2
6.3 ± 1.7
12.0 ± 5.1
8.2 ± 3.5
7.6 ± 4.0
3.8 ± 3.1
6.3 ± 4.2
4.8 ± 3.0
0.5 ± 3.0
8.5 ± 3.4
-0.6 ± 3.1
120.2 ± 14.5
100
9.1 ± 2.3
11.2 ± 2.6
7.7 ± 3.1
9.1 ± 3.4
8.4 ± 4.7
7.3 ± 3.9
8.5 ± 2.0
6.6 ± 2.8
10.7 ± 4.1
6.0 ± 2.7
7.8 ± 5.1
3.7 ± 3.3
4.9 ± 3.5
5.7 ± 4.2
-1.5 ± 3.5
10.9 ± 5.4
0.3 ± 4.7
116.4 ± 16.7
360
5.3 ± 3.4∗∗
10.8 ± 2.6
7.7 ± 2.3
9.9 ± 1.0
9.3 ± 2.8
7.5 ± 2.8
7.4 ± 3.4
5.7 ± 2.7
13.8 ± 4.0
6.6 ± 2.5
4.7 ± 2.3
4.8 ± 2.6
4.9 ± 3.2
3.1 ± 3.0
0.7 ± 2.8
6.7 ± 2.6
1.3 ± 3.1
110.2 ± 12.0
720
0.8 ± 3.3∗∗
8.4 ± 3.6∗∗
8.1 ± 2.1
10.3 ± 2.9
8.7 ± 2.5
9.0 ± 1.6∗∗
6.4 ± 1.7
7.0 ± 2.4
8.5 ± 2.5
7.4 ± 2.0
4.9 ± 2.4
3.1 ± 2.0
5.1 ± 2.1
2.7 ± 1.8
-1.6 ± 2.7
6.5 ± 2.6
1.8 ± 2.9
97.2 ± 9.0∗∗
= 15 in control and 720 mg/kg bw/day groups; in all other groups = 10. p < 0.05, ∗∗p < 0.01, statistical significances were determined with Duncan's multiple range test or with Mann–Whitney test versus control.
(b) Summary of mean body weight gain in the recovery period
Group (mg/kg bw/day)
Body weight gain (g) between days
89–96
96–103
103–110
110–117
89–117
Males
Control
12.0 ± 2.7
9.4 ± 3.6
9.2 ± 3.5
5.0 ± 3.1
35.6 ± 3.0
720
8.0 ± 4.1
15.8 ± 4.1
12.8 ± 5.9
11.4 ± 5.1
48.0 ± 12.6
Females
Control
0.6 ± 2.2
3.0 ± 3.5
5.0 ± 1.6
2.2 ± 4.3
10.8 ± 4.4
720
0.2 ± 4.5
2.4 ± 2.3
2.4 ± 7.1
6.4 ± 5.9
11.4 ± 3.8
= 5 for all groups. p < 0.05, ∗∗p < 0.01, statistical significances were determined with Duncan's multiple range test or with Mann–Whitney test versus control.