Mutagenicity and Acute Oral Toxicity Test for Herbal Poultry Feed Supplements

Table 1

Cytotoxicity test results.

EGMAX				FEED-X				Kolin PLUS				STODI
Treatment (μg/ml)	S9	Average OD ()	% of reduction	Treatment (μg/ml)	S9	Average OD ()	% of reduction	Treatment (μg/ml)	S9	Average OD ()	% of reduction	Treatment (μg/ml)	S9	Average OD ()	% of reduction
NC		0.335	NA	NC		0.391	NA	NC		0.396	NA	NC		0.409	NA
		0.475	NA			0.455	NA			0.417	NA			0.428	NA
VC		0.259	NA	VC		0.317	NA	VC		0.318	NA	VC		0.325	NA
		0.361	NA			0.385	NA			0.387	NA			0.326	NA
78.1		0.229	12	39.2		0.308	3	19.1		0.322	0	39.2		0.322	1
		0.332	8			0.355	8			0.364	6			0.332	0
156.2		0.187	28	78.1		0.326	0	39.0		0.235	26	78.1		0.330	0
		0.333	8			0.403	0			0.425	0			0.377	0
312.5		0.132	49	156.2		0.353	0	78.1		0.191	40	156.2		0.338	0
		0.315	13			0.407	0			0.408	0			0.347	0
625		0.111	57	312.5		0.332	0	156.2		0.336	0	312.5		0.273	16
		0.236	35			0.378	2			0.413	0			0.342	0
1250		0.113	57	625		0.302	5	312.5		0.372	0	625		0.223	31
		0.165	54			0.330	14			0.414	0			0.319	2
2500		0.100	61	1250		0.252	20	625		0.364	0	1250		0.167	49
		0.167	54			0.276	28			0.306	21			0.317	3

Cytotoxic concentration which showed >50% reduction in cell viability over the control. No reduction on cell viability over control. NA: not applicable; NC: negative control; VC: vehicle control (DMSO-4%).