Histological, Genotoxic, and Biochemical Effects on Cnesterodon decemmaculatus (Jenyns 1842) (Cyprinodontiformes, Poeciliidae): Early Response Bioassays to Assess the Impact of Receiving Waters

Table 2

Quantification of liver damage: values of the lesions induced by the different treatments. Data are expressed as mean ± SEM. Treatments: NC (negative control, MHW); RR (surface water of the Reconquista River); RR+Cd (surface water of the Reconquista River with 2 mg Cd/L).

Lesions	NC	RR	RR+Cd
Melanomacrophage centers	0.76 ± 0.25 (21)	0.67 ± 0.28 (18)	0.30 ± 0.17 (20)
Hyperemia	3.05 ± 0,47 (21)	2.89 ± 0.40 (18)	3.10 ± 0.64 (20)
Hemorrhages	0.76 ± 0.25 (21)	0.17 ± 0.12 (18)	0.45 ± 0.21 (20)
Hepatocytes with pyknotic nuclei	11.4  ± 1.46 (21)	37.2  ± 4.14 (18)	123.2  ± 7.34 (20)

Number of microscopic fields examined is given in parentheses. Different letters indicate significant differences among treatments (p < 0.05).
Number of individuals analyzed in each treatment: 17-20.