Research Article

Herbicide Widespread: The Effects of Pethoxamid on Nonalcoholic Fatty Liver Steatosis In Vitro

Figure 2

Oil red staining showed an increased steatosis at low-dose exposure. (a) Oil red O staining pictures for HepG2 cells in presence of fatty acids (FA) 6 mM, and pethoxamid-treated cells at different concentrations (0.1–500 ppm). (b) Spectophotometric quantification of lipid amount percentage with respect to the control of steatotic cells (FA 6 mM). t-test analyses were performed to compare the significance of each treatment with respect to CTR. Values are means ± SD from three separate experiments.