Comparison of In Vitro Endocrine Activity of Phthalates and Alternative Plasticizers

Figure 6

Antiandrogenic activity of phthalates DEHP and DINP in MDA-kb2 cells. (a, b) MDA-kb2 cells were exposed for 20–24 h to different concentrations of DEHP or DINP together with 0.25 nM DHT in 2 independent assays. Results represent the mean relative luciferase activity compared to the solvent control with 0.25 nM DHT (=100%) ±SD of 3 replicates in the 1st (a) or 2nd assay (b). (c) Competitive assay with increasing DHT concentrations. MDA-kb2 cells were exposed for 20–24 h to a single concentration of DEHP (10–4 M) or DINP (10–3 M) concomitantly with increasing concentrations of DHT. Solvent (SC), reference antiandrogen hydroxyflutamide (HF), and reference cytotoxic carbonyl cyanide 3-chlorophenylhydrazone (CCCP) were used as controls. Results represent the mean ± SD of 3 replicates.

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