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Journal of Tropical Medicine
Volume 2011 (2011), Article ID 579518, 6 pages
http://dx.doi.org/10.1155/2011/579518
Research Article

Andrographolide: A Novel Antimalarial Diterpene Lactone Compound from Andrographis paniculata and Its Interaction with Curcumin and Artesunate

1NVBDCP (Orissa State), Bhubaneswar 751001, India
2Regional Advisor, Vector-Borne Disease Control, World Health Organization, Regional Office for South East Asia, World Health House, Indraprastha Estate, Mahatma Gandhi Marg, New Delhi 110 002, India
3Institute of Life Sciences, Nalco Square, Chandrasekharpur, Bhubaneswar 751 023, Orissa, India

Received 21 September 2010; Revised 16 January 2011; Accepted 31 January 2011

Academic Editor: Sasithon Pukrittayakamee

Copyright © 2011 Kirti Mishra et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Andrographolide (AND), the diterpene lactone compound, was purified by HPLC from the methanolic fraction of the plant Andrographis paniculata. The compound was found to have potent antiplasmodial activity when tested in isolation and in combination with curcumin and artesunate against the erythrocytic stages of Plasmodium falciparum in vitro and Plasmodium berghei ANKA in vivo. IC50s for artesunate (AS), andrographolide (AND), and curcumin (CUR) were found to be 0.05, 9.1 and 17.4 μM, respectively. The compound (AND) was found synergistic with curcumin (CUR) and addictively interactive with artesunate (AS). In vivo, andrographolide-curcumin exhibited better antimalarial activity, not only by reducing parasitemia (29%), compared to the control (81%), but also by extending the life span by 2-3 folds. Being nontoxic to the in vivo system this agent can be used as template molecule for designing new derivatives with improved antimalarial properties.