Dengue Virus Entry as Target for Antiviral Therapy
Figure 4
Antiviral activity of HHA in DC-SIGN and L-SIGN transfected cell lines. (a) U87, U87/DC-SIGN, and U87/L-SIGN cells were infected with DENV-2 in the presence or absence of HHA. DENV-2 infection was analyzed by confocal microscopy using specific DENV antibodies as previously described [62]. The cell nucleus is shown in blue (Dapi) and DENV-2 infected cells are shown in green (anti-DENV Ab + Alexa 488). (b) Raji/L-SIGN cells were infected with DENV-2 in the presence or absence of HHA, and viral infection was analyzed by flow cytometry using specific DENV antibodies. The open histogram represents uninfected cells. The upper left red histogram shows the virus control (VC), and the mean fluorescence of intensity (MFI) is indicated in each panel. HHA was added at different concentrations (400–80–16 nM), and the MFI shows the dose-dependent inhibition of the DENV-2 infection in Raji/L-SIGN cells. Comparable antiviral activity of HHA in Raji/DC-SIGN cells was obtained [61].
