Journal of Tropical Medicine / 2012 / Article / Fig 2

Research Article

Reorganization of Extracellular Matrix in Placentas from Women with Asymptomatic Chagas Disease: Mechanism of Parasite Invasion or Local Placental Defense?

Figure 2

Detection of T. cruzi in human placental chorionic villi from infected mothers. The presence of parasites in villous stroma of chorionic villi from T. cruzi infected placentas was shown by immunofluorescence, detected using mAb 25 antibodies. In (a–d), noninfected chorionic villi (controls) are shown. Panels (a–d) and (e–h) show images of the same fields. Panels (a) and (e): phase contrast; (b) and (f): nuclear staining with DAPI; (c) and (g): detection of the parasite by immunofluorescence; (d) and (h): merged images of (b-c) and (f-g), respectively. Inserts show an amplified region of the detection of the parasite by immunofluorescence (g-h). Bar scale (a–d): 50 μm; (e–h): 25 μm.

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