Expression, Purification, and Evaluation of Diagnostic Potential and Immunogenicity of a Recombinant NS3 Protein from All Serotypes of Dengue Virus
Figure 1
Obtaining recombinant NS3 protein. (a) Cloning strategy for the NS3 domain protease of dengue virus in the cloning vector and in prokaryotic expression vector. (b) Amplification of the NS3 protease gene cloned into pCR2.1TOPO, (M) size marker, Lane 1, NS3-DEN1. Lane 2, NS3-DEN2. Lane 3, NS3-DEN3. Lane 4, NS3-DEN4. (c) Purification of the recombinant protein GSTNS3 of each serotype. (M) molecular weight marker. Lane 1, GST-NS3DEN1. Lane 2, GST-NS3DEN2. Lane 3, GST-NS3DEN3. Lane 4, GST-NS3DEN4. Lane 5, GST.
