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Journal of Tropical Medicine
Volume 2012, Article ID 960329, 5 pages
Research Article

Molecular Typing of Dengue Virus Circulating in Kolkata, India in 2010

ICMR Virus Unit, Kolkata, ID & BG Hospital Campus 57, Dr. S. C. Banerjee Road, Beliaghata, Kolkata 700010, India

Received 7 September 2011; Revised 19 October 2011; Accepted 20 October 2011

Academic Editor: Aditya Prasad Dash

Copyright © 2012 Arindam Sarkar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Dengue is one of the major public health threats in Kolkata. Every year, blood samples with dengue-like illness are referred to us from different medical colleges and hospitals in Kolkata for the detection of dengue infection in them. In 2010, a total of 378 samples were referred to us for that purpose. All the samples were tested for the detection of IgM antibodies by ELISA method, followed by RT-PCR test for the detection of serotypes. Only 173 samples were ELISA positive. Out of 378 samples, 108 were RT-PCR positive. Out of 108 samples, 74 samples had monotypic infection with different serotypes of DENV and 33 samples had dual infections with DENV-2 and DENV-3. Only one sample had the infection with DENV-1, DENV-2, and DENV-3. DHF was found mainly among the patients, infected with multiple dengue serotypes. Only 3 dengue monotypic infected patients had suffered from DHF.