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Journal of Tropical Medicine
Volume 2014, Article ID 159343, 6 pages
Research Article

Evaluation of the In Vitro and In Vivo Antioxidant Potentials of Aframomum melegueta Methanolic Seed Extract

1Department of Veterinary Physiology, Pharmacology, Biochemistry and Animal Health and Production, College of Veterinary Medicine, Micheal Okpara University of Agriculture, PMB 7267, Umudike, Nigeria
2Department of Biochemistry, College of Natural Sciences, Micheal Okpara University of Agriculture, PMB 7267, Umudike, Nigeria

Received 22 February 2014; Revised 1 May 2014; Accepted 4 May 2014; Published 15 May 2014

Academic Editor: Shyam Sundar

Copyright © 2014 Samuel Okwudili Onoja et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Aframomum melegueta Schum (Zingiberaceae) is a perennial herb widely cultivated for its valuable seeds in the tropical region of Africa. The present study evaluated the antioxidant effects of methanolic seed extract of A. melegueta. The antioxidant effects were evaluated using in vitro, 2, 2-diphenylpicrylhydrazine photometric assay and in vivo serum catalase, superoxide dismutase and thiobarbituric acid reactive substance assay method. The extract (25–400 μg/mL concentration) produced concentration dependent increase in antioxidant activity in 2, 2-diphenylpicrylhydrazine photometric assay. The extract (400 mg/kg) showed a significant ( ) increase in serum catalase and superoxide dismutase activity when compared with the control group. The extract (400 mg/kg) showed a significant ( ) decrease in the serum level of thiobarbituric acid reactive substance when compared with the control group. These findings suggest that the seed of A. melegueta has potent antioxidant activity which may be responsible for some of its reported pharmacological activities and can be used as antioxidant supplement.