Research Article

Concomitant Infection with Leishmania donovani and L. major in Single Ulcers of Cutaneous Leishmaniasis Patients from Sudan

Figure 3

Phylogenetic tree of COII cloning sequences. Phylogenetic tree based on a fragment of 570 bp of COII gene of Leishmania parasite. Sequence dataset was analyzed using MEGA 5, the neighbor-joining (NJ) method, and bootstrap analysis (1,000 replicates) based on the ClustalW algorithm. Significant bootstrapping values (>70%) are shown on the nodes. Blue square: colonies numbers 1 and 6 as representative sequences of 108 cytochrome oxidase II cloned sample; purple triangle: colonies numbers 1, 2, and 3 as representative sequences of 109 cytochrome oxidase II cloned sample; dark red lozenge: colonies numbers 6, 7, and 22 as representative sequences of 110 cytochrome oxidase II cloned sample; red circle: Leishmania GenBank reference sequence (L. donovani GenBank accession number AY660023; L. major GenBank accession number EF633106; L.mexicana GenBank accession number HQ586845.1 and our positive control L.infantum MHOM/TN/80/IPT1).
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