Molecular Characterization of<i> Cryptosporidium</i> spp. in Wild Rodents of Southwestern Iran Using 18s rRNA Gene Nested-PCR-RFLP and Sequencing Techniques

<div>(a) PCR-RFLP products with<i> SspI </i>restriction enzyme. Three cuttings in locations 108, 258, and 421 bp are visible on gel electrophoresis. (b) PCR-RFLP products with<i> VspI </i>restriction enzyme. Three cuttings in locations 104, 106, and 600 bp are visible on agarose gel. Lane M, DNA size marker. Lane 1, positive control for<i> Cryptosporidium</i>. Lanes 2–4, positive<i> Cryptosporidium </i>samples.</div>

Journal of Tropical Medicine

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Figure 2: Molecular Characterization of<i> Cryptosporidium</i> spp. in Wild Rodents of Southwestern Iran Using 18s rRNA Gene Nested-PCR-RFLP and Sequencing Techniques 

Figure 2 | Molecular Characterization of Cryptosporidium spp. in Wild Rodents of Southwestern Iran Using 18s rRNA Gene Nested-PCR-RFLP and Sequencing Techniques 