Antimalarial Properties of Aqueous Crude Extracts of Gynostemma pentaphyllum and Moringa oleifera Leaves in Combination with Artesunate in Plasmodium berghei-Infected Mice

Somsak, Voravuth; Borkaew, Preeyanuch; Klubsri, Chokdee; Dondee, Kittiyaporn; Bootprom, Panatda; Saiphet, Butsarat

doi:https://doi.org/10.1155/2016/8031392

Journal of Tropical Medicine

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Abstract Introduction Materials and Methods Results Discussion Conclusions Appendix Acknowledgments References Copyright Related Articles

Research Article | Open Access

Volume 2016 | Article ID 8031392 | https://doi.org/10.1155/2016/8031392

Antimalarial Properties of Aqueous Crude Extracts of Gynostemma pentaphyllum and Moringa oleifera Leaves in Combination with Artesunate in Plasmodium berghei-Infected Mice

Voravuth Somsak,¹Preeyanuch Borkaew,¹Chokdee Klubsri,¹Kittiyaporn Dondee,¹Panatda Bootprom,¹and Butsarat Saiphet¹

Academic Editor: Sukla Biswas

Received09 Aug 2016

Accepted20 Oct 2016

Published31 Oct 2016

Abstract

Due to the emergence and spread of malaria parasite with resistance to antimalarial drugs, discovery and development of new, safe, and affordable antimalarial are urgently needed. In this respect, medicinal plant extracts are targets to optimize antimalarial actions and restore efficacy of standard antimalarial drugs. The present study was aimed at determining the antimalarial activities of Gynostemma pentaphyllum and Moringa oleifera leaf extracts in combination with artesunate against Plasmodium berghei-infected mice. P. berghei ANKA maintained by serial passage in ICR mice were used based on intraperitoneal injection of 1 × 10⁷ parasitized erythrocytes and subsequent development of parasitemia. These infected mice were used to investigate the antimalarial activity of artesunate (6 mg/kg) in combination with 500, 1,000, and 2,000 mg/kg of G. pentaphyllum and M. oleifera leaf extracts using 4-day suppressive test. It was found that these extracts showed significant () antimalarial activity in dose-dependent manner with percentage of suppression of 45, 50, and 55% for G. pentaphyllum leaf extract and 35, 40, and 50% for M. oleifera leaf extract. Additionally, artesunate combined with these extracts presented higher antimalarial activity, compared to extract treated alone with percentage of suppression of 78, 91, and 96% for G. pentaphyllum leaf extract and 73, 82, and 91% for M. oleifera leaf extract. The results indicated that combination treatment of G. pentaphyllum or M. oleifera leaf extracts with artesunate was able to increase the antimalarial activity by using low dose of artesunate. Hence, these results justified the combination of these extracts and artesunate in antimalarial herbal remedies.

1. Introduction

Malaria is a major parasitic disease with high mortality and morbidity, especially in the sub-Saharan Africa, Latin America, and Asia. About 3 billion people worldwide are exposed annually, with 1.2 billion at high risk, and some 200 million developed symptomatic malaria. Moreover, it was estimated that 1 million deaths have occurred in the world [1]. This disease is caused by malarial protozoa parasite from genus Plasmodium and transmitted by female Anopheles mosquito. Due to the lack of effective vaccine to prevent malaria, the global strategy for malaria mainly focuses on case management through provision of antimalarial drugs which are capable of reducing or eliminating malaria parasites. Unfortunately, malaria parasite has developed resistance to drugs used in malarial therapy except the artemisinin [2]. However, artemisinin produces fast recrudescence when used alone due to its short half-life. Hence, artemisinin used in combination with other antimalarials, a combination known as artemisinin-combination therapy (ACT), has been recommended [3]. In addition, resistance of mosquitoes to insecticides has led to an increase in severe malaria, complicating the eradication of the disease, and the resurgence of malaria [4]. Moreover, many antimalarials in use today have high toxicity that exposes patients’ health expenditure [5]. In this respect, medicinal plant extracts are potential targets [6]. Although up to 80% of Thailand population uses traditional medicinal plants for management of diseases, these plants are not yet fully explored [7]. Various researches have been conducted to investigate the antimalarial efficacy of traditionally used plants in Asia and Africa. For example, Ramazani et al. worked on ten Iranian plant species but only Boerhavia elegans, Solanum surattense, and Prosopis juliflora showed a promising antimalarial activity in vitro and in vivo with no toxicity [8]. Verma et al. reported that Holarrhena antidysenterica and Viola canescens exhibited in vitro antimalarial activity [9].

Gynostemma pentaphyllum and Moringa oleifera have been used traditionally for treatment of several diseases involving oxidative stress and infection [10, 11]. Many reports have described the activities of these plant extracts, including antioxidant, anti-inflammation, anticancer, antiparasitic, antidiabetes, antibacterial activities, and homeostasis of biomolecules in blood [12–16]. Recently, these plant extracts were reported to exert antimalarial activity in mouse model [17]. However, combination therapy with standard antimalarial drug, artesunate, has not yet been studied using these plant extracts. Therefore, the aim of the present study is to investigate antimalarial activities of G. pentaphyllum and M. oleifera extracts in combination with artesunate in P. berghei-infected mice.

2. Materials and Methods

2.1. Artesunate

The pure artesunate powder used in this study was a gift from Dr. Chairat Uthaipibull, National Center for Genetic Engineering and Biotechnology (BIOTEC). It was dissolved in 7% Tween 80 and 3% ethanol in distilled water to provide dose of 6 mg/kg. This dose was based on the ED50 of artesunate on P. berghei-infected mice [18].

2.2. Plant Materials

Dried leaves of G. pentaphyllum and M. oleifera were purchased from the Royal Project Foundation shop at Suphan Buri province. The plant materials were identified by Dr. Sakaewan Ounjaijean at Faculty of Pharmacology, Payap University, and voucher specimens were deposited in Department of Clinical Chemistry, Faculty of Medical Technology, Western University, with voucher numbers CC-MT-WTU01 (G. pentaphyllum) and CC-MT-WTU02 (M. oleifera).

2.3. Preparation of Aqueous Crude Extracts

For preparation of crude extract, microwave-associated hot water extract was carried out as previously described [19]. Briefly, dried leaves of plant materials were ground using electric blender to obtain the powdered dried plant materials and subsequently dissolved in distilled water at the proportion of 1 : 10. The mixtures were heated in microwave at 360 W for 5 min and incubated at room temperature for overnight to obtain complete extraction. Filter through Whatman no. 1 filter paper was done, and filtrate was then freeze-dried to obtain aqueous crude extracts of G. pentaphyllum (GPE) and M. oleifera (MOE). The extracts were stored at −20°C until used. Before use, the extracts were dissolved in a mixture of 7% Tween 80 and 3% ethanol in distilled water to provide appropriate doses.

2.4. Experimental Mice

Pathogen-free female ICR mice, 4 weeks old, weighting 20–25 g, obtained from the National Laboratory Animal Center, Mahidol University, Thailand, were used. They were kept at the animal room with temperature within 25–28°C and 12 h light/12 h dark cycle. They were fed with standard diet pellet CP082 and drinking water ad libitum. All experiments involving animals were approved and ratified by the Animal Ethical Committee, Western University.

2.5. Acute Toxicity Test

GPE and MOE were evaluated for their acute toxicity in experimental mice as previously described with some modification [20]. Briefly, the mice were starved for 3-4 h before the experiment with only drinking water allowed and 1-2 h after the administration of the extracts. In the evaluation for each extract, 25 mice were randomly divided into 5 groups of 5 mice/group and were given orally 500, 1,000, 2,000, and 4,000 mg/kg body weight in single dose volume of 0.2 mL of the extracts. The control group received 0.2 mL of respective vehicle of each extract (7% Tween 80 and 3% ethanol in distilled water). Then, these mice were observed continuously for 1 h, intermittently for 4 h, and for a period of 24 h for any gross behavioral changes including rigidity, sleep, mortality, and other signs of toxicity, and follow-up continued for 30 days.

2.6. Rodent Malaria Parasite

Drug sensitive-Plasmodium berghei strain ANKA (PbANKA) obtained from MR4 (Malaria Research and Reference Reagent Resource Center, EBI Resources, University Boulevard, Manassas, VA, USA) was used in this study. The cryopreservation stock was injected intraperitoneally to naïve ICR mice. Parasitemia was determined by microscopy of Giemsa stained thin blood smear. The parasite was maintained by serial passage of 1 × 10⁷ parasitized erythrocytes on a weekly basis. Percent parasitemia was calculated using the following formula:

2.7. In Vivo Antimalarial Activity

The efficacy test of GPE, MOE, and combination with artesunate was carried out using standard protocol following Peter’s 4-day suppressive test [21]. Groups of naïve ICR mice (5 mice/group) were inoculated intraperitoneally with 1 × 10⁷ parasitized erythrocytes of PbANKA. Then, GPE (500, 1,000, and 2,000 mg/kg), MOE (500, 1,000, and 2,000 mg/kg), combination of GPE (500, 1,000, and 2,000 mg/kg) and artesunate (6 mg/kg), and combination of MOE (500, 1,000, and 2,000 mg/kg) and artesunate (6 mg/kg) were administered orally to the individual groups. The control groups were also used including artesunate (6 mg/kg) treated and untreated mice as positive and negative controls, respectively. Treatment was started 3 h after infection on day 0 and then continued daily (every 24 h) as a single dose for 4 days (days 0–3). On day 4, parasitemia was estimated by microscopy of Giemsa thin blood smear, and suppression percentage was subsequently calculated using the formula given below:

2.8. Statistical Analysis

GraphPad Prism software was used to analyze this study. All results were expressed as mean ± standard error of mean (SEM). One-way ANOVA with Tukey post hoc test was used to compare several treated groups, and significant differences were considered at 95% confidence, .

3. Results

3.1. Acute Toxicity Test

The GPE and MOE administered orally in a single dose of up to 4,000 mg/kg showed no lethal effect within 24 h of observation. Gross physical and behavioral observation of these experimental mice revealed no visible signs of toxicity such as paw licking, salivation, stretching, urination, lacrimation, hair erection, and reduction in feeding activity. Generally, the mice were physically active. Additionally, no mortality occurred within the observation period of 30 days.

3.2. Antimalarial Activity of G. pentaphyllum

The effect of GPE on PbANKA infected mice, parasitemia measurement obtained from 4-day suppressive test, and percentage of suppression of parasitemia by GPE were summarized in Figure 1. Accordingly, 500, 1,000, and 2,000 mg/kg doses of GPE significantly () inhibited parasitemia by 45, 50, and 55%, respectively, in a dose-dependent manner. The reference drug, artesunate (6 mg/kg), caused 60% suppression, which was higher than that of the extract treated groups. For combination treatment, the results indicated that GPE performed better antimalarial activity when combined with artesunate. The combination caused more significant () suppression in parasitemia burden with 78, 91, and 96%, at 6 mg/kg of artesunate combined with 500, 1,000, and 2,000 mg/kg of GPE, respectively.

Figure 1

Antimalarial activity of Gynostemma pentaphyllum. Groups of naïve ICR mice (5 mice each) were infected intraperitoneally with 1 × 10⁷ parasitized erythrocytes of PbANKA. They were then given orally the extract (500, 1,000, and 2,000 mg/kg) either alone or in combination with artesunate (6 mg/kg) for 4 consecutive days. Untreated and artesunate treated mice were used as negative and positive controls, respectively. Parasitemia and suppression percentage were subsequently measured. , and , compared to untreated control. UN: untreated control; GPE: G. pentaphyllum extract; ART: 6 mg/kg of artesunate; A + 500: combination of 6 mg/kg of artesunate and 500 mg/kg of GPE; A + 1000: combination of 6 mg/kg of artesunate and 1000 mg/kg of GPE; A + 2000: combination of 6 mg/kg of artesunate and 2000 mg/kg of GPE. The results came from 3 independent experiments.

3.3. Antimalarial Activity of M. oleifera

As shown in Figure 2, the percentage of suppression at the doses of 500, 1,000, and 2,000 mg/kg of MOE was 35, 40, and 50%, respectively, while that of artesunate treated group was 60%. The extract caused significant () suppression in a dose-dependent manner, compared to untreated control. In addition, combination of MOE with artesunate showed enhancement in antimalarial activity in a dose-dependent manner with significant (). The percentage of suppression at the doses of 6 mg/kg of artesunate combined with 500, 1,000, and 2,000 mg/kg of MOE was 73, 82, and 91%, respectively. Hence, antimalarial activity of MOE combined with artesunate was greater than the effect of artesunate or selected doses of the MOE when used singly in PbANKA infected mice.

Figure 2

Antimalarial activity of Moringa oleifera. Groups of naïve ICR mice (5 mice each) were infected intraperitoneally with 1 × 10⁷ parasitized erythrocytes of PbANKA. They were then given orally the extract (500, 1,000, and 2,000 mg/kg) either alone or in combination with artesunate (6 mg/kg) for 4 consecutive days. Untreated and artesunate treated mice were used as negative and positive controls, respectively. Parasitemia and suppression percentage were subsequently measured. , , and , compared to untreated control. UN: untreated control; MOE: M. oleifera extract; ART: 6 mg/kg of artesunate; A + 500: combination of 6 mg/kg of artesunate and 500 mg/kg of MOE; A + 1000: combination of 6 mg/kg of artesunate and 1000 mg/kg of MOE; A + 2000: combination of 6 mg/kg of artesunate and 2000 mg/kg of MOE. The results came from 3 independent experiments.

4. Discussion

P. berghei-infected mice as rodent malaria model has been employed in this study. Several standard antimalarial drugs have been identified using this rodent model; therefore, it was the appropriate malarial model for the study [22–27]. Additionally, in vivo models are usually employed in antimalarial studies because they take into account the possible effect of prodrug and probable involvement of immune system in eradication of the parasite [28]. Moreover, the choice of 4-week-old mice for this study was used to avoid the effect of anemia in old mice and the effect of possible physiological changes associated with aging-related effects on the treatment result [29].

The present study assessed the antimalarial properties of two plant extracts, G. pentaphyllum and M. oleifera, individually and in combination with artesunate. The choice of these plants was based on the remarkable activity of quinine and the success of artemisinin [30]. However, reported cases of antimalarial resistance to these drugs made the search and development of new antimalarial drugs. The aqueous crude extracts of G. pentaphyllum and M. oleifera leaves showed high antimalarial properties with dose-dependent manner against P. berghei infection in mice as evidenced by the percent inhibition of parasitemia development. These extracts exhibited a dose-dependent manner. As the dose increased, likewise the antimalarial activity increased significantly. It has been described that plant extracts contain active compounds that have great potential for medicinal use, and both traditional healers and pharmaceutical drug companies make use of these plant extracts. The extracts of G. pentaphyllum and M. oleifera leaves were reported to contain different classes of active compounds such as tannins, alkaloids, quinines, saponins, flavonoids, polyphenols, terpenoids, quercetin, kaempferol, and gypenoside [31, 32]. Terpenoids, flavonoids, alkaloids, quinines, quercetin, and kaempferol were known to have antimalarial activity [33–36]. Moreover, polyphenol and flavonoids in these extracts which have antioxidant activity may also contribute to the antimalarial activity [37]. It has been described that antioxidant activity can inhibit hemozoin formation, and free heme is very toxic for the malaria parasite [38]. These active compounds which are found in the extracts may be acting singly or in synergy with other compounds to exert the antimalarial activity. In addition, quercetin, kaempferol, and gypenoside were found evidently on the ring stage of the parasite [39]. The mode of action corresponds with the nucleic acid and protein synthesis. Furthermore, anti-inflammation of quercetin, kaempferol, and gypenoside includes the nuclear transcription factor-kappa B (NF-κB) inhibition, making therapeutic target for cancer treatment. The role of NF-κB is also important in malaria growth and development [40]. The deciphering of antimalarial property in these extracts lays the foundation for the need to reevaluate their possible role in this transcription factor regulation for malarial control.

There are several reports on antimalarial drug combination showing their in vitro and in vivo on malaria parasites [41–45]. The aqueous crude extracts of G. pentaphyllum and M. oleifera leaves exhibited high level of antimalarial activity in P. berghei-infected mice when administered with artesunate. The significant decrease in parasitemia levels in response to combination treatment compared to the control, observed with the mice, was the main finding in this study. Hence, the addictive effect of these extracts with artesunate is important in the context that offers opportunities to further standardize new artemisinin-based combination therapy as possible antimalarial combination [46].

The aqueous crude extracts of G. pentaphyllum and M. oleifera leaves were well tolerated by the mice up to the dose level of 4,000 mg/kg within 24 h and up to 30 days. In acute toxicity, doses higher than 5,000 mg/kg are generally not considered as dose related, which is in accordance with the Organization for Economic Corporation and Development (OECD) Guidance Document for Acute Oral Toxicity Testing [47]. The oral median dose of these extracts were estimated to be greater than 4,000 mg/kg which was 8 times greater than the minimum effective dose of 500 mg/kg. Previous reports have shown that the median lethal dose of a test compound is 3 times more than the minimum effective dose. This compound is considered a good candidate for future studies [48]. Therefore, these aqueous crude extracts can be considered as nontoxic at acute oral administration, and the mice were safe with the different doses of these extracts administered to them.

5. Conclusions

This study has shown that aqueous crude extracts of G. pentaphyllum and M. oleifera leaves exhibited a significant antimalarial activity. Moreover, these extracts were safe at the highest tested dose of 4,000 mg/kg. Additionally, artesunate enhanced the antimalarial activity of these extracts. Hence, G. pentaphyllum and M. oleifera leaf extracts and their combination with artesunate show strong promise for development as antimalarial combination therapy.

Appendix

See Figures 1 and 2.

Competing Interests

The authors have declared that no competing interests exist.

Acknowledgments

The authors are grateful to Dr. Chairat Uthaipibull and Associate Professor Dr. Somdet Srichairatanakool for their excellent suggestion. They thankfully acknowledge the contribution of National Center for Genetic Engineering and Biotechnology (BIOTEC), particularly for the parasite strain. Moreover, They also thank the students who helped and offered support in animal experiments.

References

N. J. White, S. Pukrittayakamee, T. T. Hien, M. A. Faiz, O. A. Mokuolu, and A. M. Dondorp, “Malaria,” The Lancet, vol. 383, no. 9918, pp. 723–735, 2014.
View at: Publisher Site | Google Scholar
H. A. Antony and S. C. Parija, “Antimalarial drug resistance: an overview,” Tropical Parasitology, vol. 6, no. 1, pp. 30–41, 2016.
View at: Publisher Site | Google Scholar
D. Sinclair, B. Zani, S. Donegan, P. Olliaro, and P. Garner, “Artemisinin-based combination therapy for treating uncomplicated malaria,” The Cochrane Database of Systematic Reviews, no. 3, Article ID CD007483, 2009.
View at: Google Scholar
J. R. D. A. Silva, A. D. S. Ramos, M. Machado et al., “A review of antimalarial plants used in traditional medicine in communities in Portuguese-Speaking countries: Brazil, Mozambique, Cape Verde, Guinea-Bissau, São Tomé and Príncipe and Angola,” Memorias do Instituto Oswaldo Cruz, vol. 106, supplement 1, pp. 142–158, 2011.
View at: Publisher Site | Google Scholar
D. J. Sullivan Jr., N. Kaludov, and M. N. Martinov, “Discovery of potent, novel, non-toxic anti-malarial compounds via quantum modelling, virtual screening and in vitro experimental validation,” Malaria Journal, vol. 10, article 274, 2011.
View at: Publisher Site | Google Scholar
T. J. Schmidt, S. A. Khalid, A. J. Romanha et al., “The potential of secondary metabolites from plants as drugs or leads against protozoan neglected diseases—part I,” Current Medicinal Chemistry, vol. 19, no. 14, pp. 2128–2175, 2012.
View at: Google Scholar
A. Thiengsusuk, W. Chaijaroenkul, and K. Na-Bangchang, “Antimalarial activities of medicinal plants and herbal formulations used in Thai traditional medicine,” Parasitology Research, vol. 112, no. 4, pp. 1475–1481, 2013.
View at: Publisher Site | Google Scholar
A. Ramazani, S. Zakeri, S. Sardari, N. Khodakarim, and N. D. Djadid, “In vitro and in vivo anti-malarial activity of Boerhavia elegans and Solanum surattense,” Malaria Journal, vol. 9, article 124, 2010.
View at: Publisher Site | Google Scholar
G. Verma, V. K. Dua, D. D. Agarwal, and P. K. Atul, “Anti-malarial activity of Holarrhena antidysenterica and Viola canescens, plants traditionally used against malaria in the Garhwal region of north-west Himalaya,” Malaria Journal, vol. 10, article 20, 2011.
View at: Publisher Site | Google Scholar
Y. H. Liu, Y. F. Liu, and X. X. Guo, “Current studies on anti-endotoxic chemical components of traditional Chinese medicine in China,” Acta Pharmacologica Sinica, vol. 22, no. 12, pp. 1071–1077, 2001.
View at: Google Scholar
A. F. Abdull Razis, M. D. Ibrahim, and S. B. Kntayya, “Health benefits of Moringa oleifera,” Asian Pacific Journal of Cancer Prevention, vol. 15, no. 20, pp. 8571–8576, 2014.
View at: Publisher Site | Google Scholar
S. J. Stohs and M. J. Hartman, “Review of the safety and efficacy of Moringa oleifera,” Phytotherapy Research, vol. 29, no. 6, pp. 796–804, 2015.
View at: Publisher Site | Google Scholar
Y. Li, J. Huang, W. Lin et al., “In vitro anticancer activity of a nonpolar fraction from Gynostemma pentaphyllum (Thunb.) Makino,” Evidence-Based Complementary and Alternative Medicine, vol. 2016, Article ID 6308649, 11 pages, 2016.
View at: Publisher Site | Google Scholar
D. Gao, M. Zhao, X. Qi et al., “Hypoglycemic effect of Gynostemma pentaphyllum saponins by enhancing the Nrf2 signaling pathway in STZ-inducing diabetic rats,” Archives of Pharmacal Research, vol. 39, no. 2, pp. 221–230, 2016.
View at: Publisher Site | Google Scholar
S. H. Park, T. L. Huh, S. Y. Kim et al., “Antiobesity effect of Gynostemma pentaphyllum extract (actiponin): a randomized, double-blind, placebo-controlled trial,” Obesity, vol. 22, no. 1, pp. 63–71, 2014.
View at: Publisher Site | Google Scholar
D. Srichana, R. Taengtip, and S. Kondo, “Antimicrobial activity of Gynostemma pentaphyllum extracts against fungi producing aflatoxin and fumonisin and bacteria causing diarrheal disease,” The Southeast Asian Journal of Tropical Medicine and Public Health, vol. 42, no. 3, pp. 704–710, 2011.
View at: Google Scholar
S. Ranasinghe, R. Ansumana, J. M. Lamin et al., “Herbs and herbal combinations used to treat suspected malaria in Bo, Sierra Leone,” Journal of Ethnopharmacology, vol. 166, pp. 200–204, 2015.
View at: Publisher Site | Google Scholar
O. O. Abiodun, N. Gbimadee, and G. O. Gbotosho, “Lopinavir/ritonavir enhanced the antimalarial activity of amodiaquine and artesunate in a mouse model of Plasmodium berghei,” Journal of Chemotherapy, pp. 1–5, 2016.
View at: Publisher Site | Google Scholar
C. Rodriguez-Perez, B. Gilbert-Lopez, J. A. Mendiola, R. Quirantes-Pine, A. Segura-Carretero, and E. Ibanez, “Optimization of microwave-assisted extraction and pressurized liquid extraction of phenolic compounds from Moringa oleifera leaves by multiresponse surface methodology,” Electrophoresis, vol. 37, pp. 1938–1946, 2016.
View at: Publisher Site | Google Scholar
D. Lorke, “A new approach to practical acute toxicity testing,” Archives of Toxicology, vol. 54, no. 4, pp. 275–287, 1983.
View at: Publisher Site | Google Scholar
W. Peters, “The chemotherapy of rodent malaria, XXII. The value of drug-resistant strains of Plasmodium berghei in screening for blood schizontocidal activity,” Annals of Tropical Medicine and Parasitology, vol. 69, no. 2, pp. 155–171, 1975.
View at: Publisher Site | Google Scholar
V. Somsak, N. Polwiang, and S. Chachiyo, “In vivo antimalarial activity of Annona muricata leaf extract in mice infected with Plasmodium berghei,” Journal of Pathogens, vol. 2016, Article ID 3264070, 5 pages, 2016.
View at: Publisher Site | Google Scholar
N. Nutham, S. Sakulmettatham, S. Klongthalay, P. Chutoam, and V. Somsak, “Protective effects of Tinospora crispa stem extract on renal damage and hemolysis during Plasmodium berghei infection in mice,” Journal of Pathogens, vol. 2015, Article ID 738608, 5 pages, 2015.
View at: Publisher Site | Google Scholar
V. Somsak, S. Chachiyo, U. Jaihan, and S. Nakinchat, “Protective effect of aqueous crude extract of neem (Azadirachta indica) leaves on Plasmodium berghei-induced renal damage in mice,” Journal of Tropical Medicine, vol. 2015, Article ID 961205, 5 pages, 2015.
View at: Publisher Site | Google Scholar
S. Audomkasok, W. Singpha, S. Chachiyo, and V. Somsak, “Antihemolytic activities of green tea, safflower, and mulberry extracts during Plasmodium berghei infection in mice,” Journal of Pathogens, vol. 2014, Article ID 203154, 4 pages, 2014.
View at: Publisher Site | Google Scholar
W. Khobjai, U. Jaihan, W. Watcharasamphankul, and V. Somsak, “Protective effect of Thunbergia laurifolia extract on hemolysis during Plasmodium berghei infection,” Parasitology Research, vol. 113, no. 5, pp. 1843–1846, 2014.
View at: Publisher Site | Google Scholar
V. Somsak, U. Jaihan, S. Srichairatanakool, and C. Uthaipibull, “Protection of renal function by green tea extract during Plasmodium berghei infection,” Parasitology International, vol. 62, no. 6, pp. 548–551, 2013.
View at: Publisher Site | Google Scholar
P. J. Waako, B. Gumede, P. Smith, and P. I. Folb, “The in vitro and in vivo antimalarial activity of Cardiospermum halicacabum L. and Momordica foetida Schumch. Et Thonn,” Journal of Ethnopharmacology, vol. 99, no. 1, pp. 137–143, 2005.
View at: Publisher Site | Google Scholar
C. Pierrot, E. Adam, S. Lafitte et al., “Age-related susceptibility and resistance to Plasmodium berghei in mice and rats,” Experimental Parasitology, vol. 104, no. 1-2, pp. 81–85, 2003.
View at: Publisher Site | Google Scholar
J. Karbwang and T. Harinasuta, “Overview: clinical pharmacology of antimalarials,” The Southeast Asian Journal of Tropical Medicine and Public Health, vol. 23, supplement 4, pp. 95–109, 1992.
View at: Google Scholar
P. T. Ky, P. T. Huong, T. K. My et al., “Dammarane-type saponins from Gynostemma pentaphyllum,” Phytochemistry, vol. 71, no. 8-9, pp. 994–1001, 2010.
View at: Publisher Site | Google Scholar
G. Karthivashan, M. Tangestani Fard, P. Arulselvan, F. Abas, and S. Fakurazi, “Identification of bioactive candidate compounds responsible for oxidative challenge from hydro-ethanolic extract of Moringa oleifera leaves,” Journal of Food Science, vol. 78, no. 9, pp. C1368–C1375, 2013.
View at: Publisher Site | Google Scholar
T. Murata, T. Miyase, F. W. Muregi et al., “Antiplasmodial triterpenoids from Ekebergia capensis,” Journal of Natural Products, vol. 71, no. 2, pp. 167–174, 2008.
View at: Publisher Site | Google Scholar
A. Yenesew, H. M. Akala, H. Twinomuhwezi et al., “The antiplasmodial and radical scavenging activities of flavonoids of Erythrina burttii,” Acta Tropica, vol. 123, no. 2, pp. 123–127, 2012.
View at: Publisher Site | Google Scholar
I. C. Ezenyi, O. A. Salawu, R. Kulkarni, and M. Emeje, “Antiplasmodial activity-aided isolation and identification of quercetin-4’-methyl ether in Chromolaena odorata leaf fraction with high activity against chloroquine-resistant Plasmodium falciparum,” Parasitology Research, vol. 113, no. 12, pp. 4415–4422, 2014.
View at: Publisher Site | Google Scholar
M. Barliana, E. Suradji, R. Abdulah et al., “Antiplasmodial properties of kaempferol-3-O-rhamnoside isolated from the leaves of Schima wallichii against chloroquine-resistant Plasmodium falciparum,” Biomedical Reports, vol. 2, no. 4, pp. 579–583, 2014.
View at: Publisher Site | Google Scholar
F. El Babili, J. Bouajila, I. Fouraste, A. Valentin, S. Mauret, and C. Moulis, “Chemical study, antimalarial and antioxidant activities, and cytotoxicity to human breast cancer cells (MCF7) of Argania spinosa,” Phytomedicine, vol. 17, no. 2, pp. 157–160, 2010.
View at: Publisher Site | Google Scholar
M. Y. Lubbad, S. Al-Quraishy, and M. A. Dkhil, “Antimalarial and antioxidant activities of Indigofera oblongifolia on Plasmodium chabaudi-induced spleen tissue injury in mice,” Parasitology Research, vol. 114, no. 9, pp. 3431–3438, 2015.
View at: Publisher Site | Google Scholar
K. Mishra, A. P. Dash, B. K. Swain, and N. Dey, “Anti-malarial activities of Andrographis paniculata and Hedyotis corymbosa extracts and their combination with curcumin,” Malaria Journal, vol. 8, article 26, 2009.
View at: Publisher Site | Google Scholar
M. A. Hidalgo, A. Romero, J. Figueroa et al., “Andrographolide interferes with binding of nuclear factor-κB to DNA in HL-60-derived neutrophilic cells,” British Journal of Pharmacology, vol. 144, no. 5, pp. 680–686, 2005.
View at: Publisher Site | Google Scholar
A. O. Adepiti and E. O. Iwalewa, “Evaluation of the combination of Uvaria chamae (P. Beauv.) and amodiaquine in murine malaria,” Journal of Ethnopharmacology, vol. 193, pp. 30–35, 2016.
View at: Publisher Site | Google Scholar
A. O. Adepiti, A. A. Elujoba, and O. O. Bolaji, “Evaluation of herbal antimalarial MAMA decoction-amodiaquine combination in murine malaria model,” Pharmaceutical Biology, vol. 54, no. 10, pp. 2298–2303, 2016.
View at: Publisher Site | Google Scholar
R. Sahu, L. A. Walker, and B. L. Tekwani, “In vitro and in vivo anti-malarial activity of tigecycline, a glycylcycline antibiotic, in combination with chloroquine,” Malaria Journal, vol. 13, article 414, 2014.
View at: Publisher Site | Google Scholar
C. Desgrouas, J. Dormoi, C. Chapus, E. Ollivier, D. Parzy, and N. Taudon, “In vitro and in vivo combination of cepharanthine with anti-malarial drugs,” Malaria Journal, vol. 13, no. 1, article 90, 2014.
View at: Publisher Site | Google Scholar
O. L. Anagu, A. A. Attama, V. C. Okore, H. T. Gugu, A. A. Ngene, and C. O. Esimone, “Azadirachta indica extract-artesunic acid combination produces an increased cure rate of Plasmodium berghei-infected mice,” Pharmaceutical Biology, vol. 52, no. 7, pp. 883–889, 2014.
View at: Publisher Site | Google Scholar
J. Pousibet-Puerto, J. Salas-Coronas, A. Sánchez-Crespo et al., “Impact of using artemisinin-based combination therapy (ACT) in the treatment of uncomplicated malaria from Plasmodium falciparum in a non-endemic zone,” Malaria Journal, vol. 15, article 339, 2016.
View at: Publisher Site | Google Scholar
A. Hayes, Guidelines for Acute Oral Toxicity Testing, Raven Press, New York, NY, USA, 2nd edition, 1987.
E. Schleyer, S. Kuhn, H. Ruhrs et al., “Oral idarubicin pharmacokinetics-correlation of trough level with idarubicin area under curve,” Leukemia, supplement 5, no. 11, pp. S15–S21, 1997.
View at: Google Scholar

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Copyright © 2016 Voravuth Somsak et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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