Diversity of Leptospira spp. in Rats and Environment from Urban Areas of Sarawak, Malaysia

Table 2

Nucleotide BLAST results showing similarity with LipL32 gene of pathogenic Leptospira, 16S rRNA gene of intermediate Leptospira,and rrs gene of saprophytic Leptospira from GenBank.

Sample ID	Sample	Accession number	Description	Maximum score	Total score	Query coverage	Maximum identity
Pathogenic
CFP4	Rat	KC800993.1	Leptospira interrogans serovar Icterohaemorrhagiae strain RTCC 2812 outer membrane protein (lipL32) gene, partial cds	525	525	99%	89%
CFP5	Rat	KC800993.1	Leptospira interrogans serovar Icterohaemorrhagiae strain RTCC 2812 outer membrane protein (lipL32) gene, partial cds	486	486	99%	87%
CFP39	Rat	AY461920.1	Leptospira noguchii strain LT796 LipL32 (lipL32) gene, partial cds	512	512	97%	88%
CFP40	Rat	AY461920.1	Leptospira noguchii strain LT796 LipL32 (lipL32) gene, partial cds	486	486	94%	90%
CFP24	Soil	KF297610.1	Leptospira weilii clone lipL32-122069 LipL32 gene, partial cds	462	462	72%	91%
CFP25	Soil	AY461920.1	Leptospira noguchii strain LT796 LipL32 (lipL32) gene, partial cds	510	510	94%	88%
CFP29	Soil	AY461924.1	Leptospira noguchii strain LT796 LipL32 (lipL32) gene, partial cds	286	286	99%	75%
CFP32	Soil	AY461920.1	Leptospira noguchii strain LT796 LipL32 (lipL32) gene, partial cds	521	521	97%	89%
CFP37	Soil	AY461920.1	Leptospira noguchii strain LT796 LipL32 (lipL32) gene, partial cds	542	542	94%	90%
CFP34	Soil	AY609333.1	Leptospira borgpetersenii serovar Mini major outer membrane protein (lipl32) gene, complete cds	508	508	93%	88%
CFP35	Soil	AY609333.1	Leptospira borgpetersenii serovar Mini major outer membrane protein (lipl32) gene, complete cds	518	518	98%	89%
CFP36	Soil	AY461920.1	Leptospira noguchii strain LT796 LipL32 (lipL32) gene, partial cds	514	514	97%	88%
CFP7	Water	AY609333.1	Leptospira borgpetersenii serovar Mini major outer membrane protein (lipl32) gene, complete cds	510	510	99%	88%
CFP9	Water	JN886739.1	Leptospira interrogans serovar Hardjo strain RTCC2821 Lipl32 (lipl32) gene, partial cds	494	494	99%	88%
CFP28	Water	JN886739.1	Leptospira interrogans serovar Hardjo strain RTCC2821 Lipl32 (lipl32) gene, partial cds	336	336	99%	77%
CFP31	Water	CP006723.1	Leptospira interrogans serovar Linhai str. 56609 chromosome 1, complete sequence	525	525	93%	89%
Intermediate
CFG16	Soil	NR_044042.1	Leptospira wolffii serovar Khorat strain Khorat-H2 16S ribosomal RNA gene, partial sequence	562	562	99%	97%
CFG7	Water	NR_044042.1	Leptospira wolffii serovar Khorat strain Khorat-H2 16S ribosomal RNA gene, partial sequence	592	592	97%	99%
CFG17	Water	AY631891.1	Leptospira inadai serovar Aguaruna strain MW 4 16S ribosomal RNA gene, partial sequence	545	545	97%	96%
CFG19	Water	NR_044042.1	Leptospira wolffii serovar Khorat strain Khorat-H2 16S ribosomal RNA gene, partial sequence	584	584	98%	99%
Saprophytic
CFS15	Rat	KP739778.1	Leptospira meyeri strain 19CAP 16S ribosomal RNA gene, partial sequence	431	431	98%	98%
CFS16	Rat	KP739778.1	Leptospira meyeri strain 19CAP 16S ribosomal RNA gene, partial sequence	425	425	98%	98%
CFS17	Rat	KP739778.1	Leptospira meyeri strain 19CAP 16S ribosomal RNA gene, partial sequence	388	388	96%	95%
CFS18	Rat	KP739778.1	Leptospira meyeri strain 19CAP 16S ribosomal RNA gene, partial sequence	353	353	91%	99%
CFS19	Rat	KP739778.1	Leptospira meyeri strain 19CAP 16S ribosomal RNA gene, partial sequence	446	446	99%	99%
CFS36	Rat	KP739778.1	Leptospira meyeri strain 19CAP 16S ribosomal RNA gene, partial sequence	416	416	98%	97%
CFS4	Soil	JQ988852.1	Leptospira meyeri strain Semaranga_DB49 16S ribosomal RNA gene, partial sequence	433	433	98%	98%
CFS21	Soil	KP739778.1	Leptospira meyeri strain 19CAP 16S ribosomal RNA gene, partial sequence	418	418	98%	97%
CFS20	Water	KP739778.1	Leptospira meyeri strain 19CAP 16S ribosomal RNA gene, partial sequence	412	412	98%	96%