Development and Validation of a PCR-ELISA for the Diagnosis of Symptomatic and Asymptomatic Infection by Leishmania (Leishmania) infantum

Figure 2

Analytical sensitivity of conventional PCR and kDNA PCR-ELISA in spiked blood samples. (a) Analytical sensitivity of 6% polyacrylamide gels and (b) kDNA PCR-ELISA for the detection of PCR products from DNA extracted from spiked blood samples with the addition of 1 parasite (lines 1 and 2) and 10 (lines 3 and 4), 100 (lines 5 and 6), 1,000 (lines 7, 8, and 9), and 10,000 parasites (lines 10, 11, and 12); PC: positive control, NC: negative control, and M: 100 pb DNA Ladder (Promega). The absorbance readings correspond to the mean of the duplicate for each parasite concentration.

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