Lateral Flow Loop-Mediated Isothermal Amplification Test with Stem Primers: Detection of Cryptosporidium Species in Kenyan Children Presenting with Diarrhea

Figure 2

The detection of stem LAMP product from some selected reactions done for over 60 minutes using 2.0% agarose gels stained with ethidium bromide, SYBR Green 1 dye, and LFD dipstick format. 1 = (false positive), 2 = C. hominis, 3 = C. parvum, and NC = PCR water. (b) The appearance of nonspecific products at 75 minutes’ reaction cut-off time. 1, M044 (false positive), 2, M099 (false positive), and NC = PCR water. TL = test line; CL = control line. The nonspecific products show different patterns agarose gel and turn green on addition of SYBR Green 1. However, none was positive using the LFD format.

(a)

(b)