Journal of Transplantation / 2012 / Article / Fig 2

Research Article

c-Jun-N-Terminal Kinase Signaling Is Involved in Cyclosporine-Induced Epithelial Phenotypic Changes

Figure 2

JNK signaling is involved in cyclosporine-induced EPCs. (a) Cellular morphology after 48 h exposure to 6 μM cyclosporine with or without 2 μM (L)-JNKI1 was determined using phase contrast microscopy. Bar, 4 μm. (b). Cell viability was determined using the MTS assay after 72 h exposure to various concentrations of cyclosporine (CsA) with or without 2 μM (L)-JNKI1; * 𝑃 = 0 . 0 4 , 𝑛 = 4 . (c) Real-time PCR analysis of E-cadherin mRNA expression after 48 h exposure to various concentrations of CsA with or without 2 μM (L)-JNKI1. (d) Representative western blot of E-cadherin in whole cell lysates after exposure to vehicle, 6 μM CsA, 2 μM (L)-JNKI1, and 6 μM CsA + 2 μM (L)-JNKI1 for 48 hours. (e) Representative phase contrast micrographs of the initial wounding and 12 hours after wound healing (left). Bar, 50 μm.