Inflammation-Mediated Regulation of MicroRNA Expression in Transplanted Pancreatic Islets

Table 3

SAM of miRNAs expressed in transplanted syngeneic islets collected 3 days after implantation. MiRNA expression in transplanted islets was compared to control isolated islets. 26 common miRNAs that displayed altered expression in vivo and in vitro (cytokine treated islets) are shown in bold font. MiRNA confirmed by PCR are marked with an X in the last column. FI and SD are fold increase and standard deviation of hybridization values (transplanted islets versus control islets), respectively.

Upregulated miRNAs	(𝑑)	𝑞-(%)	FI	SD	PCR
miR-21	7.75	0.00	26.58	13.21	X
miR-98	4.40	0.00	7.94	3.60	X
miR-212	4.33	0.00	51.61	43.25
miR-27a	4.15	0.00	19.56	14.07	X
rno-let-7i	4.13	0.00	16.16	15.27
miR-207	4.01	0.00	20.62	15.18
miR-143	3.94	0.00	9.12	6.07	X
rno-let-7a	3.89	0.00	13.03	9.55
rno-let-7d	3.89	0.00	7.20	3.30	X
miR-126	3.83	0.00	41.18	60.44	X
miR-195	3.68	0.00	8.12	5.00	X
miR-22	3.66	0.00	10.14	8.45	X
miR-27b	3.54	0.00	10.57	6.85
miR-107	3.49	0.00	8.16	5.90
miR-99b	3.38	0.00	5.01	2.15
miR-214	3.34	0.00	10.52	7.15	X
miR-125b-5p	3.33	0.00	8.13	4.95
rno-let-7e	3.20	0.00	3.76	1.37
miR-23a	3.17	0.00	9.24	8.21
rno-let-7b	2.95	0.00	5.27	3.79
miR-24	2.93	0.00	7.61	6.34
miR-30e	2.91	0.00	7.77	4.92
miR-23b	2.87	0.00	7.37	6.20
miR-26a	2.80	0.00	5.93	3.69
miR-29c	2.77	0.00	6.97	4.10
miR-375	2.74	0.00	6.04	3.89
Downregulated miRNAs	(𝑑)	𝑞-(%)	FI	SD	PCR
miR-542-5p	−6.03	0.00	19.82	11.24
miR-129	−5.09	0.00	25.58	25.01	X
miR-326	−3.03	0.00	5.89	4.12	X
miR-370	−2.85	0.00	4.78	1.87
miR-484	−2.55	0.00	11.70	2.74