|
| Bank number | Processing media | Antibiotic regimen | Incubation protocol | Cryopreservation method | Storage condition and duration |
|
| N1 | Heart recovery, transport, and dissection solution: Ringer's lactate
Antibiotics media: DMEM with Hepes
Cryopreservation media: DMEM + 7.5% DMSO | Vancomycin: 50 ug/mL
Gentamicin: 80 ug/mL
Cefoxitin: 240 ug/mL | Until the 28th of June 2010: 1°C–10°C, 22–26 hours
Present: 33°C–38°C, 18–26 hours | Controlled rate freezing | No response provided |
|
| N2 | Antibiotics media: RPMI-1640
Cryopreservation media: X-VIVO-10 + DMSO | Cefoxitin,
colymycin-M,
vancomycin,
lincomycin | 4°C, 24 hours | Controlled rate freezing | Liquid nitrogen vapour phase: 5 years |
|
| N3 | Transport and storage solution:
Hanks solution
Dissection solution: saline
Cryopreservation media: 10% DMSO | Gentamicin: 80 mg/mL
Cefazolin or Kefzol: 1 mg/mL | 4°C, 24 hours | Controlled rate freezing | Ultralow temperature (−140°C): 5 years |
|
| N4 | Transport and processing solution: Ringer's lactate
Cryopreservation media: saline + RPMI-1640 + RPMI 1640 with 10% FBS + DMSO | Cefoxitin: 240 ug/mL
Polymyxin B: 100 mg/mL
Vancomycin: 50 ug/mL
Lincomycin: 120 ug/mL
(soon to use gentamicin) | 1°C–10°C, 22–26 hours | Controlled rate freezing | Liquid nitrogen vapour phase: 5 years |
|
| N5 | DMEM
Cryopreservation media:
cardiac tissue—DMEM + DMSO + FBS
vascular tissue—DMEM + DMSO + chondroitin + FBS | Two-stage process:
1st cocktail to achieve primary decontamination,
2nd cocktail to support tissue | Warm solutions, >24 hours | Controlled rate freezing | Liquid nitrogen vapour phase: 5 years |
|
| N6 | RPMI-1640 | Vancomycin: 50 ug/mL
Colymycin M: 75 mg/mL
Cefoxitin: 100 mg/mL
Lincomycin: 300 mg/mL | 4°C, hours | Controlled rate freezing | Liquid nitrogen vapour phase: 5 years |
|
