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Journal of Transplantation
Volume 2016, Article ID 2106028, 8 pages
http://dx.doi.org/10.1155/2016/2106028
Research Article

C1Q Assay Results in Complement-Dependent Cytotoxicity Crossmatch Negative Renal Transplant Candidates with Donor-Specific Antibodies: High Specificity but Low Sensitivity When Predicting Flow Crossmatch

1Department of Transplantation, National Institute of Medical Sciences and Nutrition Salvador Zubirán, Vasco de Quiroga 15, Colonia Belisario Domínguez, Sección XVI, Tlalpan, 14080 Mexico City, Mexico
2Histocompatibility Laboratory, National Institute of Medical Sciences and Nutrition Salvador Zubirán, Vasco de Quiroga 15, Colonia Belisario Domínguez, Sección XVI, Tlalpan, 14080 Mexico City, Mexico
3Department of Nephrology-Mineral Metabolism, National Institute of Medical Sciences and Nutrition Salvador Zubirán, Vasco de Quiroga 15, Colonia Belisario Domínguez, Sección XVI, Tlalpan, 14080 Mexico City, Mexico

Received 29 March 2016; Revised 22 June 2016; Accepted 13 July 2016

Academic Editor: Andreas Zuckermann

Copyright © 2016 José M. Arreola-Guerra et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. R. Patel and P. I. Terasaki, “Significance of the positive crossmatch test in kidney transplantation,” The New England Journal of Medicine, vol. 280, no. 14, pp. 735–739, 1969. View at Publisher · View at Google Scholar · View at Scopus
  2. R. Pei, J.-H. Lee, T. Chen, S. Rojo, and P. I. Terasaki, “Flow cytometric detection of HLA antibodies using a spectrum of microbeads,” Human Immunology, vol. 60, no. 12, pp. 1293–1302, 1999. View at Publisher · View at Google Scholar · View at Scopus
  3. E. M. Gibney, L. R. Cagle, B. Freed, S. E. Warnell, L. Chan, and A. C. Wiseman, “Detection of donor-specific antibodies using HLA-coated microspheres: another tool for kidney transplant risk stratification,” Nephrology Dialysis Transplantation, vol. 21, no. 9, pp. 2625–2629, 2006. View at Publisher · View at Google Scholar · View at Scopus
  4. R. A. Bray and H. M. Gebel, “Strategies for human leukocyte antigen antibody detection,” Current Opinion in Organ Transplantation, vol. 14, no. 4, pp. 392–397, 2009. View at Publisher · View at Google Scholar · View at Scopus
  5. A. A. Zachary, J. T. Sholander, J. A. Houp, and M. S. Leffell, “Using real data for a virtual crossmatch,” Human Immunology, vol. 70, no. 8, pp. 574–579, 2009. View at Publisher · View at Google Scholar · View at Scopus
  6. B. D. Tait, C. Süsal, H. M. Gebel et al., “Consensus guidelines on the testing and clinical management issues associated with HLA and non-HLA antibodies in transplantation,” Transplantation, vol. 95, no. 1, pp. 19–47, 2013. View at Publisher · View at Google Scholar · View at Scopus
  7. H. E. Feucht, H. Schneeberger, G. Hillebrand et al., “Capillary deposition of C4d complement fragment and early renal graft loss,” Kidney International, vol. 43, no. 6, pp. 1333–1338, 1993. View at Publisher · View at Google Scholar · View at Scopus
  8. M. Crespo, M. Pascual, N. Tolkoff-Rubin et al., “Acute humoral rejection in renal allograft recipients: I. Incidence, serology and clinical characteristics,” Transplantation, vol. 71, no. 5, pp. 652–658, 2001. View at Publisher · View at Google Scholar · View at Scopus
  9. M. Wahrmann, M. Exner, H. Regele et al., “Flow cytometry based detection of HLA alloantibody mediated classical complement activation,” Journal of Immunological Methods, vol. 275, no. 1-2, pp. 149–160, 2003. View at Publisher · View at Google Scholar · View at Scopus
  10. M. Wahrmann, M. Exner, B. Haidbauer et al., “[C4d]FlowPRA screening—a specific assay for selective detection of complement-activating anti-HLA alloantibodies,” Human Immunology, vol. 66, no. 5, pp. 526–534, 2005. View at Publisher · View at Google Scholar · View at Scopus
  11. G. Bartel, M. Wahrmann, M. Exner et al., “In vitro detection of C4d-fixing HLA alloantibodies: associations with capillary C4d deposition in kidney allografts,” American Journal of Transplantation, vol. 8, no. 1, pp. 41–49, 2008. View at Publisher · View at Google Scholar · View at Scopus
  12. M. Wahrmann, G. Bartel, M. Exner et al., “Clinical relevance of preformed C4d-fixing and non-C4d-fixing HLA single antigen reactivity in renal allograft recipients,” Transplant International, vol. 22, no. 10, pp. 982–989, 2009. View at Publisher · View at Google Scholar · View at Scopus
  13. J. D. Smith, I. M. Hamour, N. R. Banner, and M. L. Rose, “C4d fixing, luminex binding antibodies—a new tool for prediction of graft failure after heart transplantation,” American Journal of Transplantation, vol. 7, no. 12, pp. 2809–2815, 2007. View at Publisher · View at Google Scholar · View at Scopus
  14. G. Honger, M. Wahrmann, P. Amico, H. Hopfer, G. A. Böhmig, and S. Schaub, “C4d-fixing capability of low-level donor-specific HLA antibodies is not predictive for early antibody-mediated rejection,” Transplantation, vol. 89, no. 12, pp. 1471–1475, 2010. View at Publisher · View at Google Scholar
  15. M. Wahrmann, M. Exner, M. Schillinger et al., “Pivotal role of complement-fixing HLA alloantibodies in presensitized kidney allograft recipients,” American Journal of Transplantation, vol. 6, no. 5, pp. 1033–1041, 2006. View at Publisher · View at Google Scholar · View at Scopus
  16. G. Chen, F. Sequeira, and D. B. Tyan, “Novel C1q assay reveals a clinically relevant subset of human leukocyte antigen antibodies independent of immunoglobulin G strength on single antigen beads,” Human Immunology, vol. 72, no. 10, pp. 849–858, 2011. View at Publisher · View at Google Scholar · View at Scopus
  17. G. Chen, F. Sequeira, and D. Tyan, “A new combo-flow crossmatch (CFMX) for simultaneous detection of IgG and complement fixing antibodies (CFABS),” Abstracts/Human Immunology, vol. 74, pp. 1–49, 2013, Abstract 7-OR. View at Google Scholar
  18. A. M. Patel, C. Pancoska, S. Mulgaonkar, and F. L. Weng, “Renal transplantation in patients with pre-transplant donor-specific antibodies and negative flow cytometry crossmatches,” American Journal of Transplantation, vol. 7, no. 10, pp. 2371–2377, 2007. View at Publisher · View at Google Scholar · View at Scopus
  19. A. Gupta, V. Iveson, M. Varagunam, S. Bodger, P. Sinnott, and R. C. Thuraisingham, “Pretransplant donor-specific antibodies in cytotoxic negative crossmatch kidney transplants: are they relevant?” Transplantation, vol. 85, no. 8, pp. 1200–1204, 2008. View at Publisher · View at Google Scholar · View at Scopus
  20. N. L. Reinsmoen, C.-H. Lai, A. Vo et al., “Acceptable donor-specific antibody levels allowing for successful deceased and living donor kidney transplantation after desensitization therapy,” Transplantation, vol. 86, no. 6, pp. 820–825, 2008. View at Publisher · View at Google Scholar · View at Scopus
  21. L. Couzi, C. Araujo, G. Guidicelli et al., “Interpretation of positive flow cytometric crossmatch in the era of the single-antigen bead assay,” Transplantation, vol. 91, no. 5, pp. 527–535, 2011. View at Publisher · View at Google Scholar · View at Scopus
  22. R. J. Duquesnoy, Y. Awadalla, J. Lomago et al., “Retransplant candidates have donor-specific antibodies that react with structurally defined HLA-DR,DQ,DP epitopes,” Transplant Immunology, vol. 18, no. 4, pp. 352–360, 2008. View at Publisher · View at Google Scholar · View at Scopus
  23. M. C. S. Freitas, L. M. Rebellato, M. Ozawa et al., “The role of immunoglobulin-G subclasses and C1q in de novo HLA-DQ donor-specific antibody kidney transplantation outcomes,” Transplantation, vol. 95, no. 9, pp. 1113–1119, 2013. View at Google Scholar · View at Scopus
  24. D. Redondo-Pachón, J. Pascual, M. J. Pérez-Sáez et al., “Impact of preformed and de novo anti-HLA DP antibodies in renal allograft survival,” Transplant Immunology, vol. 34, pp. 1–7, 2016. View at Publisher · View at Google Scholar · View at Scopus
  25. E. J. Lepin and E. F. Reed, “Complement-independent mechanisms of antigraft antibodies in transplant arteriosclerosis and accommodation,” Current Opinion in Organ Transplantation, vol. 9, no. 1, pp. 10–15, 2004. View at Publisher · View at Google Scholar · View at Scopus
  26. P. T. Jindra, Y.-P. Jin, E. Rozengurt, and E. F. Reed, “HLA class I antibody-mediated endothelial cell proliferation via the mTOR pathway,” Journal of Immunology, vol. 180, no. 4, pp. 2357–2366, 2008. View at Publisher · View at Google Scholar · View at Scopus
  27. J. Lion, C. Taflin, A. R. Cross et al., “HLA class II antibody activation of endothelial cells promotes Th17 and disrupts regulatory T lymphocyte expansion,” American Journal of Transplantation, vol. 16, no. 5, pp. 1408–1420, 2016. View at Publisher · View at Google Scholar
  28. F. Eskandary, G. Bond, N. Kozakowski et al., “Diagnostic contribution of donor-specific antibody characteristics to uncover late silent antibody-mediated rejection-results of a cross-sectional screening study,” Transplantation, 2016. View at Publisher · View at Google Scholar