Comparison of Oral Tolerance to ApoB and HSP60 Peptides in Preventing Atherosclerosis Lesion Formation in Apob48−/Ldlr− Mice
Effect of tolerance on cytokine production. (a) Concentration of the cytokines (TNF-α, IFN-γ, and IL-10) in the plasma was determined by ELISA as per the manufacturers instructions, three days after the last dose and at the end of the study after 10 weeks of high fat diet feed to induce atherosclerosis following oral administration of ApoB and Hsp60 peptides (). (b) Splenocytes isolated from orally treated animals at the end of the study were stimulated with ApoB or Hsp60 peptide (10 μg/mL) in vitro for 72 hrs. Cytokine concentrations (TNF-α, IFN-γ TGF-β, and IL-10) in the supernatant were estimated by ELISA. () , , .