Table of Contents
Journal of Vaccines
Volume 2013 (2013), Article ID 618324, 8 pages
http://dx.doi.org/10.1155/2013/618324
Research Article

Immunogenicity and Protective Efficacy of a Polyvalent DNA Vaccine against Human Orthopoxvirus Infections Based on Smallpox Virus Genes

State Research Center of Virology and Biotechnology VECTOR, Koltsovo, Novosibirsk 630559, Russia

Received 26 May 2012; Accepted 11 July 2012

Academic Editor: Barbara Schnierle

Copyright © 2013 Rinat A. Maksyutov et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

DNA vaccines combining plasmids carrying the variola virus genes M1R, A30L, and F8L of intracellular virion surface membrane proteins as well as A36R and B7R of the extracellular virus envelope proteins under control of Rous sarcoma virus or cytomegalovirus promoters have been constructed. These DNA vaccines induced production of a high titers of vaccinia virus-neutralizing antibodies in mice similar to those elicited by the live vaccinia virus immunization. Mice vaccinated by created DNA vaccine were completely protected against a lethal (10 LD50) challenge with highly pathogenic ectromelia virus. These results suggest that such vaccine should be efficient in immunization of humans against smallpox.