Evolution of New Variants/Mutants of JE Virus, Its Effect on Neurovirulence, Antigenicity, Host Immune Responses and Disease Transmission in Endemic Areas
Table 2
Theoretical prediction of formation of most possible single site revertants (mutations) through recombination and its effect on neurovirulence.
E protein
Residue(s) in clusters
Recombination
Level of neurovirulence
Amino acid
Epitopic region
Within the group
Out of the group
M−/M+ Ratio
% Rec.
Increase/decrease
1 2 3 4 5 (M+)*
1.0
1.0
1.0
1.0
Similar to PS
6 7 8 9 10 (M−)
1.0
1.0
1.0
1.0
Similar to PS
1 2 3 6 5
0.266
1.36
0.195
1.3
↑ < PS
1 2 6 4 5
0.337
1.50
0.224
1.2
↑ < PS
1 6 3 4 5
0.367
1.58
0.232
1.1
↑ < PS
6 2 3 4 5
0.388
1.636
0.237
1.1
↑ < PS
1 2 3 4 6
0.414
1.70
0.243
1.0
↑ < PS
1 2 3 4 7
0.303
1.436
0.211
1.26
↑ < PS
1 2 3 7 5
0.375
1.60
0.234
1.10
↑ < PS
1 2 7 4 5
0.421
1.72
0.244
1.00
↑ < PS
1 7 3 4 5
0.450
1.81
0.248
1.00
↑ < PS
7 2 3 4 5
0.466
1.87
0.249
0.97
↓ > PS
1 2 3 4 8
0.414
1.70
0.243
1.06
↑ < PS
1 2 3 8 5
0.466
1.87
0.249
0.97
↓ > PS
1 2 8 4 5
0.500
2.00
0.250
0.90
↓ > PS
1 8 3 4 5
0.521
2.09
0.249
0.86
↓ > PS
8 2 3 4 5
0.533
2.14
0.249
0.84
↓ > PS
1 2 3 4 9
0.504
2.01
0.250
0.90
↓ > PS
1 2 3 9 5
0.375
1.60
0.234
0.83
↓ > PS
1 2 9 4 5
0.566
2.30
0.246
0.78
↓ > PS
1 9 3 4 5
0.583
2.40
0.242
0.75
↓ > PS
9 2 3 4 5
0.592
2.45
0.241
0.74
↓ > PS
1 2 3 4 10
0.75
2.36
0.317
0.769
↓ > PS
1 2 3 10 5
0.84
2.52
0.333
0.719
↓ > PS
1 2 10 4 5
0.91
2.65
0.343
0.684
↓ > PS
1 10 3 4 5
0.96
2.74
0.350
0.662
↓ > PS
10 2 3 4 5
0.99
2.80
0.353
0.649
↓ > PS
Prediction of formation of most possible multiple site revertants through recombination and its effect on neurovirulence
1 2 3 4 5
1.0
1.0
1.0
1.0
↑ < PS
1 6 3 6 5
0.367
1.58
0.232
1.14
↑ < PS
1 2 6 4 6
0.408
1.69
0.241
1.07
↑ < PS
1 6 3 4 6
0.438
1.78
0.240
1.02
↑ < PS
1 6 2 6 5
0.636
1.85
0.343
0.98
↑ > PS
6 2 3 6 5
1.0
2.0
0.50
0.90
↑ > PS
6 2 3 4 6
0.455
1.83
0.248
0.90
↑ > PS
1 6 6 4 5
0.517
2.07
0.249
0.877
↑ > PS
6 2 6 4 5
0.529
2.12
0.249
0.854
↑ > PS
7 2 3 7 5
0.595
2.47
0.240
0.840
↑ > PS
1 7 3 4 7
0.556
2.25
0.245
0.806
↑ > PS
1 7 2 7 5
0.570
2.32
0.245
0.781
↑ > PS
1 7 3 7 5
0.586
2.36
0.248
0.787
↑ > PS
7 2 3 4 7
0.566
2.30
0.246
0.751
↑ > PS
1 2 7 4 7
0.537
2.16
0.248
0.735
↑ > PS
1 7 7 4 5
0.607
2.54
0.238
0.714
↑ > PS
7 2 7 4 5
0.615
2.60
0.236
0.699
↑ > PS
1 2 8 4 8
0.630
2.70
0.233
0.671
↑ > PS
1 8 2 8 5
0.651
2.87
0.266
0.632
↑ > PS
1 8 3 4 8
0.642
2.82
0.227
0.649
↑ > PS
8 2 3 4 8
0.649
2.85
0.227
0.636
↑ > PS
1 8 3 8 5
0.662
2.96
0.223
0.613
↑ > PS
8 2 3 8 5
0.668
3.01
0.221
0.602
↑ > PS
1 8 8 4 5
0.766
3.09
0.247
0.588
↑ > PS
8 2 8 4 5
0.682
3.14
0.217
0.578
↑ > PS
1 2 9 4 9
0.699
3.32
0.210
0.546
↑ > PS
1 9 3 4 9
0.707
3.41
0.207
0.531
↑ > PS
9 2 3 4 9
0.270
3.47
0.207
0.523
↑ > PS
1 9 2 9 5
0.713
3.49
0.204
0.508
↑ > PS
1 9 3 9 5
0.712
3.58
0.198
0.507
↑ > PS
9 2 3 9 5
0.725
3.63
0.199
0.500
↑ > PS
1 9 9 4 5
0.730
3.70
0.197
0.490
↑ > PS
9 2 9 4 5
0.738
3.818
0.193
0.476
↑ > PS
1 10 3 10 5
0.765
4.27
0.179
0.425
↑ > PS
1 10 10 4 5
0.757
4.4
0.172
0.423
↑ > PS
10 2 3 10 5
0.768
4.3
0.187
0.420
↑ > PS
10 2 10 4 5
0.775
4.45
0.174
0.408
↑ > PS
1 2 10 4 10
0.751
4.01
0.187
0.452
↑ > PS
1 10 3 4 10
0.756
4.10
0.184
0.442
↑ > PS
10 2 3 4 10
0.759
4.16
0.182
0.436
↑ > PS
1 10 3 10 5
0.765
4.27
0.179
0.425
↑ > PS
↑ < PS Increasing but higher than the parental strain, ↑ > PS increasing but lower than parental strain. (1) All sequential changes in antigenic sites with in the group = Sncv − Snv/Sncv. Total no. of shifts in antigenic sites in circulating virus, No. of probable shifts generated in newly emerged virus. (2) All sequential changes in antigenic sites out of the group = Sncv − Snv/Spv. No. of sequences in antigenic sites in parental virus. (3) Possible % recombination frequency in mutants/variants of JE virus = 1/Square values of total shifts due to recombination in new progeny virus X100. (4) Number of sequences in antigenic sites of M+ and M− recombining viruses are 5. (5) Possible alternate triplet codons 3, amino acids 2. (6) Revertants mutations occur at single and more than two sites.
