Analogs of LDL Receptor Ligand Motifs in Dengue Envelope and Capsid Proteins as Potential Codes for Cell Entry

Figure 4

Dual label HeLa cell binding experiments using Dsp2EP-FITC and Apo E-CM-DiI. Overlay of Dsp2EP-FITC (green fluorescence) and Apo E-CM-DiI (red fluorescence), frame (A), shows colocalized signals in yellow. Images of areas labeled 1–8 of the overlay image were enlarged in Photoshop then analyzed with Image J. Image 1 shows diffused signal for Dsp2EP-FITC (green) with clusters of red signal indicating Apo E-CM-DiI. Images in frames 1–8 reveal a consistent pattern, that is, diffused (spread) binding of Dsp2EP-FITC and clustering of Apo E-CM-DiI. Red and green colors were separated using Image J and then rendered using Interactive 3D Surface Plot Plugin algorithm. Images 1G and 1R represent green and red signals, respectively. Images 1R, 2R, 5R, and 6R represent Apo E-CM-DiI and show formation of clusters of similar intensity. Corresponding green signal images show less uniformity.