Table of Contents
Journal of Viruses
Volume 2015, Article ID 646303, 15 pages
Research Article

Analogs of LDL Receptor Ligand Motifs in Dengue Envelope and Capsid Proteins as Potential Codes for Cell Entry

Biophysics Research Laboratory, Department of Physics and Astronomy, University of Texas at Brownsville, One West University Boulevard, Brownsville, TX 78520, USA

Received 9 December 2014; Revised 17 March 2015; Accepted 17 March 2015

Academic Editor: Tatiana Betakova

Copyright © 2015 Juan Guevara Jr. et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Basic amino acid clusters in motifs, XBBBXXBX, XBBXBX, and ΨBΨXB, in apo E and apo B100 of the low-density lipoproteins, are known to bind the LDL receptors, heparin, and highly sulfated polysaccharides also occur in the polyproteins sequences of the Dengue viruses 1-4. The ΨBΨXB motif occurs in Domain III, the putative receptor binding region of the DENV envelope glycoproteins, twice in DENV 1 and 2, and once in DENV 3 and 4. The DENV2 synthetic peptide Dsp2EP-FITC that encompasses residues 0564Gly-Gly0595 in the envelope protein region of the polyprotein and DENV3 Dsp3CP-FITC, 0002Asn-Gln0028, in the N-terminal capsid region of the DENV3 polyprotein were used to assess binding to Hep G2 cells and Liposorber Dextran Sulfate Cellulose Affinity beads of the Lipopheresis System.

  1. Supplementary Material