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Journal of Veterinary Medicine
Volume 2013, Article ID 125096, 8 pages
http://dx.doi.org/10.1155/2013/125096
Research Article

Impact of Seminal Chemical Elements on the Oxidative Balance in Bovine Seminal Plasma and Spermatozoa

1Department of Animal Physiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia
2Department of Animal Physiology and Health, Szent István University, Páter Karoly utca 1, Gödöllő 2103, Hungary
3Institute of Biology, Faculty of Geography and Biology, Pedagogical University of Krakow, ul. Podbrzezie 3, 31 045 Kraków, Poland

Received 28 April 2013; Revised 22 July 2013; Accepted 7 August 2013

Academic Editor: Daniel A. Feeney

Copyright © 2013 Eva Tvrdá et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Mutual relationships between selected chemical elements (Na, K, Fe, Cu, Mg, and Zn), basic motility characteristics (motility and progressive motility), and markers of the oxidative balance (superoxide dismutase, catalase, glutathione, albumin, and malondialdehyde) were investigated in bovine seminal plasma and spermatozoa. Computer assisted sperm analysis was used to assess the motility parameters; mineral concentrations were determined by the voltammetric method and flame absorption spectrophotometry; antioxidants and malondialdehyde were evaluated by UV/VIS spectrophotometry. Concentrations of chemical elements in both seminal fractions were in the following descending order: Na > K > Zn > Mg > Fe > Cu. Higher amounts of all minerals and nonenzymatic antioxidants were detected in the seminal plasma ( ; ), while higher MDA concentration and activity of enzymatic antioxidants were recorded in the cell lysates ( ; ). Na, Fe, Cu, Mg, and Zn were positively correlated with the motility and antioxidant parameters ( ; ; ). Inversely, K exhibited the positive associations with malondialdehyde ( ). This study demonstrates that most chemical elements are integral components of bovine semen and are needed for the protection against oxidative stress development.