Journal of Veterinary Medicine / 2013 / Article / Tab 2

Research Article

Impact of Seminal Chemical Elements on the Oxidative Balance in Bovine Seminal Plasma and Spermatozoa

Table 2

Chemical and biochemical parameters of bovine seminal plasma and spermatozoa examined by the ASV and FAS methods (chemical elements) as well as UV/VIS spectrophotometry (prooxidant and antioxidant markers) ( ).

ParameterSeminal plasmaCell lysates

SOD [U/mg protein]0.49 ± 0.030.60 ± 0.05
CAT [U/mg protein]1.19 ± 0.102.01 ± 0.14**
GSH [mg/mg protein]0.10 ± 0.020.01 ± 0.002***
ALB [mg/mg protein]0.18 ± 0.030.10 ± 0.005**
MDA [µM]2.94 ± 0.1913.29 ± 0.67***
Na [mg/dL]179.44 ± 7.01141.11 ± 3.02**
K [mg/dL]25.97 ± 2.1021.57 ± 1.15
Fe [mg/dL]4.15 ± 0.542.02 ± 0.30**
Cu [mg/dL]2.39 ± 0.271.15 ± 0.15**
Mg [mg/dL]7.65 ± 0.902.03 ± 0.19**
Zn [mg/dL]23.59 ± 2.059.40 ± 1.10**

Mean ± SEM. **P < 0.01; ***P < 0.001. SOD: superoxide dismutase; CAT: catalase; GSH: glutathione; ALB: albumin; MDA: malondialdehyde; Na: sodium; K: potassium; Fe: iron; Cu: copper; Mg: magnesium; Zn: zinc.

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