Impact of Seminal Chemical Elements on the Oxidative Balance in Bovine Seminal Plasma and Spermatozoa

Table 2

Chemical and biochemical parameters of bovine seminal plasma and spermatozoa examined by the ASV and FAS methods (chemical elements) as well as UV/VIS spectrophotometry (prooxidant and antioxidant markers) (𝑛=30).

Parameter	Seminal plasma	Cell lysates
SOD [U/mg protein]	0.49 ± 0.03	0.60 ± 0.05
CAT [U/mg protein]	1.19 ± 0.10	2.01 ± 0.14**
GSH [mg/mg protein]	0.10 ± 0.02	0.01 ± 0.002***
ALB [mg/mg protein]	0.18 ± 0.03	0.10 ± 0.005**
MDA [µM]	2.94 ± 0.19	13.29 ± 0.67***
Na [mg/dL]	179.44 ± 7.01	141.11 ± 3.02**
K [mg/dL]	25.97 ± 2.10	21.57 ± 1.15
Fe [mg/dL]	4.15 ± 0.54	2.02 ± 0.30**
Cu [mg/dL]	2.39 ± 0.27	1.15 ± 0.15**
Mg [mg/dL]	7.65 ± 0.90	2.03 ± 0.19**
Zn [mg/dL]	23.59 ± 2.05	9.40 ± 1.10**

Mean ± SEM. **P < 0.01; ***P < 0.001. SOD: superoxide dismutase; CAT: catalase; GSH: glutathione; ALB: albumin; MDA: malondialdehyde; Na: sodium; K: potassium; Fe: iron; Cu: copper; Mg: magnesium; Zn: zinc.