Table of Contents
Laser Chemistry
Volume 19, Issue 1-4, Pages 169-172

Temperature Effects Of Excitation Laser Pulses During Step-Scan FT-IR Measurements

1Institut für Biophysikund Strahlenbiologie, Albert-Ludwigs-Universität, Albertstrasse 23, Freiburg 79104, Germany
2Department of Organic Chemistry, The Weizmann Institute of Science, Rehovot 76100, Israel

Received 6 April 1997

Copyright © 1999 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Replacing the chromophore of bacteriorhodopsin with chemically modified retinal analogs which cannot isomerize, allows to measure directly side effects from the laser pulse used for sample excitation in step-scan FT-IR measurements. Comparison with static temperature difference spectra and temperature-jump experiments shows that the observed effects can mainly be attributed to heating of the sample by the laser pulse. The size of resulting spectral changes is compared to difference bands of the photoreaction.