Assisted Interpretation of Laser-Induced Fluorescence Spectra of Egg-Based Binding Media Using Total Emission Fluorescence Spectroscopy

Nevin, Austin; Anglos, Demetrios

doi:https://doi.org/10.1155/2006/82823

Laser Chemistry

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Lasers in the Preservation of Cultural Heritage

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Research Article | Open Access

Volume 2006 | Article ID 082823 | https://doi.org/10.1155/2006/82823

Assisted Interpretation of Laser-Induced Fluorescence Spectra of Egg-Based Binding Media Using Total Emission Fluorescence Spectroscopy

Austin Nevin^1,2and Demetrios Anglos¹

Academic Editor: Marta Castillejo

Received13 Sept 2006

Revised20 Oct 2006

Accepted27 Oct 2006

Published28 Dec 2006

Abstract

Laser-induced fluorescence (LIF) spectroscopy can provide nondestructive, qualitative analysis of protein-based binding media found in artworks. Fluorescence emissions from proteins in egg yolk and egg white are due to autofluorescent aromatic amino acids as well as other native and age-related fluorophores, but the potential of fluorescence spectroscopy for the differentiation between binding media is dependent on the choice of a suitable excitation wavelength and limited by problems in interpretation. However, a better understanding of emission spectra associated with LIF can be achieved following comparisons with total emission fluorescence spectra where a series of consecutive emission spectra are recorded over a specific range. Results using nanosecond UV laser sources for LIF of egg-based binding media are presented which are rationalised following comparisons with total emission spectra. Specifically, fluorescence is assigned to tryptophan and oxidation products of amino acids; in the case of egg yolk, fatty-acid polymerisation and age-related degradation products account for the formation of fluorophores.

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Copyright

Copyright © 2006 Austin Nevin and Demetrios Anglos. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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