Changes in Cell Viability of Wounded Fibroblasts following Laser Irradiation in Broad-Spectrum or Infrared Light

Hawkins, Denise; Abrahamse, Heidi

doi:https://doi.org/10.1155/2007/71039

Laser Chemistry

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Research Article | Open Access

Volume 2007 | Article ID 071039 | https://doi.org/10.1155/2007/71039

Changes in Cell Viability of Wounded Fibroblasts following Laser Irradiation in Broad-Spectrum or Infrared Light

Denise Hawkins¹and Heidi Abrahamse¹

Academic Editor: Frederik Claeyssens

Received29 Jun 2006

Revised14 Aug 2006

Accepted22 Aug 2006

Published21 Dec 2006

Abstract

Objective. This study aimed to establish if broad-spectrum or infrared (IR) light in combination with laser therapy can assist phototherapy to improve the cell function of wounded cells. Background. The effect of laser light may be partly or completely reduced by broad-spectrum light. Methods. Wounded human skin fibroblasts were irradiated with 5 J/cm2 using a helium-neon laser, a diode laser, or an Nd:YAG laser in the dark, in the light, or in IR. Changes in cell viability were evaluated by cell morphology, ATP cell viability, LDH membrane integrity, and caspase 3/7 as an early marker of apoptosis. Results. Wounded cells exposed to 5 J/cm2 using 632.8 nm in the dark or 830 nm in the light or 1064 nm in the dark showed an increase in ATP viability, an increase in cytokine expression, and a decrease in LDH cytotoxicity indicating that the metabolic activity of the wounded cells was stimulated. Conclusion. Wounded cells irradiated in IR light showed an undesirable thermal effect that was proportional to the duration of exposure.

References

B. Shaffer, “Scientific basis of laser energy,” Clinics in Sports Medicine, vol. 21, no. 4, pp. 585–598, 2002.
View at: Publisher Site | Google Scholar
N. Bhatta, K. Isaacson, K. M. Bhatta, R. R. Anderson, and I. Schiff, “Comparative study of different laser systems,” Fertility and Sterility, vol. 61, no. 4, pp. 581–591, 1994.
View at: Google Scholar
J. P. Garino, P. A. Lotke, A. A. Sapega, P. J. Reilly, and J. L. Esterhai Jr., “Osteonecrosis of the knee following laser-assisted arthroscopic surgery: a report of six cases,” Arthroscopy, vol. 11, no. 4, pp. 467–474, 1995.
View at: Publisher Site | Google Scholar
S. J. Gitomer and R. D. Jones, “Laser-produced plasmas in medicine,” in Laser-Tissue Interaction, S. L. Jacques, Ed., vol. 1202 of Proceedings of SPIE, pp. 118–132, Los Angeles, Calif, USA, January 1990.
View at: Publisher Site | Google Scholar
T. I. Karu, G. S. Kalendo, V. S. Letokhov, and V. V. Lobko, “Biostimulation fo Hela cells by low-intensity visible light,” Il Nuovo Cimento D, vol. 1, no. 6, pp. 828–840, 1982.
View at: Publisher Site | Google Scholar
I. Stadler, R. J. Lanzafame, P. Oskoui, R.-Y. Zhang, J. Coleman, and M. Whittaker, “Alteration of skin temperature during low-level laser irradiation at 830 nm in a mouse model,” Photomedicine and Laser Surgery, vol. 22, no. 3, pp. 227–231, 2004.
View at: Publisher Site | Google Scholar
I. Greppi, “Diode laser hair removal of the black patient,” Lasers in Surgery and Medicine, vol. 28, no. 2, pp. 150–155, 2001.
View at: Publisher Site | Google Scholar
O. N. Bogacheva, K. A. Samoilova, and N. A. Zhevago et al., “Enhancement of fibroblast growth promoting activity of human blood after its irradiation in vivo (transcutaneously) and in vitro with visible and infrared polarized light,” Tsitologiia, vol. 46, no. 2, pp. 159–171, 2004.
View at: Google Scholar
J. Kubota, “Defocused diode laser therapy (830 nm) in the treatment of unresponsive skin ulcers: a preliminary trial,” Journal of Cosmetic and Laser Therapy, vol. 6, no. 2, pp. 96–102, 2004.
View at: Publisher Site | Google Scholar
H. Meffert, H. P. Scherf, and H. Bäumler et al., “Systemic effects of ultraviolet, visible and infrared radiation in serial whole body irradiation. I. Oxygen utilization, flow properties of blood, hemodynamics, blood components and phagocytosis,” Dermatologische Monatsschrift, vol. 175, no. 10, pp. 609–622, 1989.
View at: Google Scholar
S. Belotsky, R. Avtalion, M. Sinyakov, R. Lavi, and R. Lubart, “Visible light affects chemiluminescence of carp (Cyprinus carpio) blood leukocytes,” Photomedicine and Laser Surgery, vol. 22, no. 3, pp. 255–258, 2004.
View at: Publisher Site | Google Scholar
R. J. Lanzafame, I. Stadler, and J. Coleman et al., “Temperature-controlled 830-nm low-level laser therapy of experimental pressure ulcers,” Photomedicine and Laser Surgery, vol. 22, no. 6, pp. 483–488, 2004.
View at: Publisher Site | Google Scholar
Y. Maegawa, T. Itoh, T. Hosokawa, K. Yaegashi, and M. Nishi, “Effects of near-infrared low-level laser irradiation on microcirculation,” Lasers in Surgery and Medicine, vol. 27, no. 5, pp. 427–437, 2000.
View at: Publisher Site | Google Scholar
R. Ausubel, R. Brent, and R. E. Kingston et al., Short Protocols in Molecular Cloning, John Wiley & Sons, New York, NY, USA, 4th edition, 1994.
View at: Google Scholar
J. Sambrook, E. F. Fritsch, and T. Maniatis, Molecular Cloning: A laboratory Manual, Cold Spring Harbor Laboratory Press, New York, NY, USA, 2nd edition, 1989.
View at: Google Scholar
M. Kreisler, A. B. Christoffers, B. Willershausen, and B. D'Hoedt, “Low-level 809 nm GaAIAs laser irradiation increases the proliferation rate of human laryngeal carcinoma cells in vitro,” Lasers in Medical Science, vol. 18, no. 2, pp. 100–103, 2003.
View at: Publisher Site | Google Scholar
J. C. Yarrow, Z. E. Perlman, N. J. Westwood, and T. J. Mitchison, “A high-throughput cell migration assay using scratch wound healing, a comparison of image-based readout methods,” BMC Biotechnology, vol. 4, no. 1, p. 21, 2004.
View at: Publisher Site | Google Scholar
W. Posten, D. A. Wrone, J. S. Dover, K. A. Arndt, S. Silapunt, and M. Alam, “Low-level laser therapy for wound healing: mechanism and efficacy,” Dermatologic Surgery, vol. 31, no. 3, pp. 334–340, 2005.
View at: Publisher Site | Google Scholar
D. Cha, P. O'Brien, E. A. O'Toole, D. T. Woodley, and L. G. Hudson, “Enhanced modulation of keratinocyte motility by transforming growth factor- $α$ (TGF- $α$ ) relative to epidermal growth factor (EGF),” Journal of Investigative Dermatology, vol. 106, no. 4, pp. 590–597, 1996.
View at: Publisher Site | Google Scholar
D. G. Siposan and A. Lukacs, “Relative variation to received dose of some erythrocytic and leukocytic indices of human blood as a result of low-level laser radiation: an in vitro study,” Journal of Clinical Laser Medicine and Surgery, vol. 19, no. 2, pp. 89–103, 2001.
View at: Publisher Site | Google Scholar
J. Rigau, C.-H. Sun, M. A. Trelles, and M. W. Berns, “Effects of the 633-nm laser on the behavior and morphology of primary fibroblast culture,” in Effects of Low-Power Light on Biological Systems, T. I. Karu and A. R. Young, Eds., vol. 2630 of Proceedings of SPIE, pp. 38–42, Barcelona, Spain, September 1996.
View at: Publisher Site | Google Scholar
T. Riss, R. Moravec, M. Beck, R. Hannah, K. Wilson, and R. Swanson, “CellTiter- ${Glo}^{TM}$ luminescent cell viability assay: fast, sensitive and flexible,” Promega Notes Magazine, vol. 81, pp. 2–5, 2002.
View at: Google Scholar
S. P. M. Crouch, R. Kozlowski, K. J. Slater, and J. Fletcher, “The use of ATP bioluminescence as a measure of cell proliferation and cytotoxicity,” Journal of Immunological Methods, vol. 160, no. 1, pp. 81–88, 1993.
View at: Publisher Site | Google Scholar
R. Moravec, “Total cell quantitation using the CytoTox $96^{TM}$ non-radioactive cytotoxicity assay,” Promega Notes, vol. 45, pp. 11–12, 1994.
View at: Google Scholar
D. W. Nicholson and N. A. Thornberry, “Caspases: killer proteases,” Trends in Biochemical Sciences, vol. 22, no. 8, pp. 299–306, 1997.
View at: Publisher Site | Google Scholar
C. S. Enwemeka, J. C. Parker, D. S. Dowdy, E. E. Harkness, L. E. Sanford, and L. D. Woodruff, “The efficacy of low-power lasers in tissue repair and pain control: a meta-analysis study,” Photomedicine and Laser Surgery, vol. 22, no. 4, pp. 323–329, 2004.
View at: Publisher Site | Google Scholar
T. Karu, “Primary and secondary mechanisms of the action of monochromatic visible and near infrared radiation on cells,” in The Science of Low-Power Laser Therapy, p. 33, Gordon and Breach Science, Amsterdam, The Netherlands, 1998.
View at: Google Scholar
J. Tunér and L. Hode, Laser Therapy—Clinical Practice and Scientific Background, Prima Books AB, Grängesberg, Sweden, 2002.
View at: Google Scholar
M. K. Wong and A. I. Gotlieb, “The reorganization of microfilaments, centrosomes, and microtubules during in vitro small wound reendothelialization,” Journal of Cell Biology, vol. 107, no. 5, pp. 1777–1783, 1988.
View at: Publisher Site | Google Scholar
B. L. Coomber and A. I. Gotlieb, “In vitro endothelial wound repair. Interaction of cell migration and proliferation,” Arteriosclerosis, vol. 10, no. 2, pp. 215–222, 1990.
View at: Google Scholar
J.-M. Zahm, H. Kaplan, and A.-L. Hérard et al., “Cell migration and proliferation during the in vitro wound repair of the respiratory epithelium,” Cell Motility and the Cytoskeleton, vol. 37, no. 1, pp. 33–43, 1997.
View at: Publisher Site | Google Scholar
T. I. Karu and S. F. Kolyakov, “Exact action spectra for cellular responses relevant to phototherapy,” Photomedicine and Laser Surgery, vol. 23, no. 4, pp. 355–361, 2005.
View at: Publisher Site | Google Scholar
D. Hawkins and H. Abrahamse, “Biological effects of Helium-Neon laser irradiation on normal and wounded human skin fibroblasts,” Photomedicine and Laser Surgery, vol. 23, no. 3, pp. 251–259, 2005.
View at: Publisher Site | Google Scholar

Copyright

Copyright © 2007 Denise Hawkins and Heidi Abrahamse. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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