Table of Contents
Laser Chemistry
Volume 2009, Article ID 474858, 14 pages
Research Article

Development, Characterization, and Application of a Versatile Single Particle Detection Apparatus for Time-Integrated and Time-Resolved Fluorescence Measurements—Part II: Experimental Evaluation

Department of Chemistry, University of Florida, Gainesville, FL 32611, USA

Received 10 February 2009; Accepted 13 April 2009

Academic Editor: Savas K. Georgiou

Copyright © 2009 Xihong Wu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This paper describes the experimental realization and characterization of a versatile single particle detection apparatus. The system utilizes a novel particle beam inlet that can serve as either an on-line particle concentrator (i.e., all diameters confined in a narrow beam) or as a segregator (i.e., selected diameters confined in a narrow beam) and can be operated in a high-speed mode as well as in a low-speed mode, thus allowing different interaction times between the particles and the laser beam. An aerodynamic sizing technique has been incorporated into the system to provide rapid, real-time, and high-resolution sizing. Parameters such as transmission efficiency and size-segregation efficiency have been measured. The performance of the instrument has been demonstrated by on-line detection of spectrally resolved and time resolved fluorescence detection from airborne dye-doped particles and aerosolized endogenous fluorophores found in biological agents.