Combination Phototherapy with a Histone Deacetylase Inhibitor and a Potent DNA-Binding Bibenzimidazole: Effects in Haematological Cell Lines

Figure 3

SAHA decreases the viability and increases apoptosis in malignant and transformed haematological cells. K562 (a), CEM-CCRF (c), R100 (e), and LCL (g) cells were treated with the indicated concentration of SAHA for 24 and 48 hours, and the cell viability was measured using the CellTiter-Glo Luminescent Cell Viability Assay kit. The data is presented as the percentage of growth inhibition relative to untreated cells. For the measurement of apoptosis, 48 hours after incubation with HDACi, the level of caspase 3/7 activity was analysed in K562 (b), CEM-CCRF (d), R100 (f), and LCL (h) cells using the Caspase-Glo 3/7 assay kit following manufacturer instructions. Results are expressed as means ± SEM, 𝑛=2. *𝑃<0.05, **𝑃<0.01, ***𝑃<0.001 as compared to untreated.

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