Table of Contents
Metal-Based Drugs
Volume 1 (1994), Issue 2-3, Pages 279-287

2D NMR Methods for Structural Delineation of Copper(II) Complexes of Penicillin and Pilocarpine

Department of Chemistry, University of Siena, Pian dei Mantellini 44, Siena I-53100, Italy

Received 30 November 1993

Copyright © 1994 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A method was developed for delineating the structure of paramagnetic metal complexes. The selective disappearance of cross-peaks in proton-carbon shift correlated 2D NMR maps was shown to uniquely depend upon the scalar and/or dipolar interaction between ligand nuclei and the unpaired electron(s), thus providing a means of identifying binding sites. Copper(II) was shown to form metal complexes with both Penicillin (PNC) and Pilocarpine (PLC) and the structure of the two 1:2 complexes in water solution at physiological pH were determined.