Table of Contents
Metal-Based Drugs
Volume 1 (1994), Issue 5-6, Pages 433-443
http://dx.doi.org/10.1155/MBD.1994.433

Transport of the Dicyanogold(I) Anion

Department of Biological Sciences and Chemistry, Laboratory of Clinical Virology and Biomedical Chemistry Research Center, University of Cincinnati, Cincinnati 45221-0006, OH, USA

Copyright © 1994 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We have shown that dicyanogold(I), [Au(CN)2]- is a common metabolite found in blood and urine samples of patients treated with different gold based drugs. Some patients have high levels of gold within their red blood cells (RBCs). Size exclusion and C18 reversed phase chromatography show that the majority of the gold in RBC lysates is bound to protein, but small molecules such as dicyanogold(I) and gold-glutathione complexes are also present. Dicyanogold incubation with red blood cells in vitro leads to a rapid and complete uptake of gold. This uptake is unaffected by DIDS, an inhibitor of the anion channel, but is blocked by the addition of external cyanide. Dicyanogold is also readily taken up by H9 cells, a continuous CD4+ cell line. This uptake is significantly inhibited by N-ethylmaleimide, suggesting a requirement for sulfhydryl groups. Dicyanogold inhibits the replication of the AIDS virus, HIV, in a cell culture model.