Table of Contents
Metal-Based Drugs
Volume 2008, Article ID 281843, 5 pages
http://dx.doi.org/10.1155/2008/281843
Research Article

Phthalocyanine-Based Molecularly Imprinted Polymers as Nucleoside Receptors

Dipartimento di Ingegneria dell'Innovazione, Università del Salento, Lecce 73100, Italy

Received 5 September 2007; Accepted 31 October 2007

Academic Editor: Jannie C. Swarts

Copyright © 2008 Luigia Longo and Giuseppe Vasapollo. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A molecularly imprinted polymer (MIP) for tri-O-acetyladenosine (TOAA), PPM(TOAA), was prepared by the combined use of methacrylic acid (MAA) and Zn(II)tetra(4'-methacryloxyphenoxy) phthalocyanine as functional monomers. This MIP exhibited a higher binding ability for TOAA compared to the MIP prepared using only MAA, PM(TOAA), in batch rebinding tests. Scatchard analysis gave a higher association constant of PPM(TOAA) for TOAA (2.96×104 M1) than that of PM(TOAA) (1.48×104 M1). The MIP prepared using only the zinc-phthalocyanine, PP(TOAA), did not show any binding capacity for TOAA. This means that the phthalocyanine in the MIP contributes to higher affinities, although it barely interacts with TOAA. Since selectivity for this kind of MIPs is more important than binding affinity, the binding of TOAA and a structurally related compound, tri-O-acetyluridine (TOAU), on the polymers was investigated. Both PPM(TOAA) and PM(TOAA) exhibited binding affinities for TOAA while they did not show any binding capacity for TOAU.