Expression of a Gene Encoding 34.9 kDa PPE Antigen of Mycobacterium avium subsp. paratuberculosis in E. coli

Table 1

DTH elicited by purified recombinant PPE protein in M. a. paratuberculosis sensitized mice (mean diameter of erythema in mm) upon recall with 10 $\mu$g of the indicated antigen.

	Antigen
Sensitizing organism	Johnin PPD	Recombinant PPE protein	PBS
M. a. paratuberculosis	3.95 $\pm$ 0.5	3.12 $\pm$ 0.4	0.18 $\pm$ 0.2
Unsensitized	0.17 $\pm$ 0.3	0.13 $\pm$ 0.2	0.13 $\pm$ 0.5