Glucose-6-Phosphate Dehydrogenase of Trypanosomatids: Characterization, Target Validation, and Drug Discovery

Table 1

T. brucei (Tb), T. cruzi (Tc), and L. mexicana (Lm) G6PDH kinetic constants for an ordered bi-bi-reaction mechanism.

Source	K G6P	K NADP^+	V1	k cat	K iG6P
	(μM)	(μM)	(nmoles of NADPH⋅s^−1)	(s^−1)	(μM)
Tb	57.8 ± 2.4	9.4 ± 0.4	36.2 ± 1.5	16.4 ± 0.6	47.6 ± 1.9
Tc	206.0 ± 4.2	22.5 ± 1.2	77.7 ± 2.5	57.1 ± 1.9	105.3 ± 4.6
Lm	74.5 ± 3.0	12.1 ± 0.5	31.2 ± 1.2	22.2 ± 0.9	86.4 ± 3.5

Data from Cordeiro et al. [45, 46].